TNF RII/TNFRSF1B Antibody

Western Blot: TNF RII/TNFRSF1B Antibody [NBP1-88139] - Analysis in human cell line CAPAN-2.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody [NBP1-88139] - Staining of human lymph node shows moderate membranous positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody [NBP1-88139] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody [NBP1-88139] - Staining of human liver shows moderate membranous positivity in hepatocytes.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody [NBP1-88139] - Staining of human stomach shows moderate membranous positivity in glandular cells.

Product Discontinued

TNF RII/TNFRSF1B Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFRSF1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24743550).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TNF RII/TNFRSF1B Antibody

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF RII/TNFRSF1B Antibody (NBP1-88139)(3)

Publications using NBP1-88139

• Dadiani M, Necula D, Kahana-Edwin S et Al. TNFR2+ TILs are significantly associated with improved survival in triple-negative breast cancer patients Cancer Immunol. Immunother. 2020-03-20 [PMID: 32198536] (IF/IHC, Human)
• Bachmann J, Burte F, Pramana S et al. Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria. PLoS Pathog 2014-04-01 [PMID: 24743550]
• Kato BS, Nicholson G, Neiman M et al. Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011-11-17 [PMID: 22093360]

FAQs for TNF RII/TNFRSF1B Antibody (NBP1-88139). (Showing 1 - 1 of 1 FAQs).

1. Is this antibody purified to IgG only. This info is needed in order to make a control with a chromopure rabbit IgG.
   • NBP1-88139 is affinity purified, so this product should only contain the purified antibody (isotype rabbit IgG) in solution (PBS, glycerol, and sodium azide).

Bioinformatics

• Gene Symbol: TNFRSF1B