TNF RII/TNFRSF1B Antibody [Unconjugated]

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Raw264.7 mouse macrophage cell line, resting (open histogram) or treated with 1 μg/mL LPS overnight (filled histogram), was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-associated Proteins protocol.

L‑929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

TNF RII/TNFRSF1B was detected in immersion fixed L‑929 mouse fibroblast cell line using Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TNF RII/TNFRSF1B Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse TNF RII/TNFRSF1B
  Val23-Gly258
  Accession # P25119
• Specificity: Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1.7-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RII/TNFRSF1B Antibody [Unconjugated]

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta . These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF RII/TNFRSF1B Antibody (AF-426-PB)(12)

Publications using AF-426-PB

• Raffaele, S;Thougaard, E;Laursen, CCH;Gao, H;Andersen, KM;Nielsen, PV;Ortí-Casañ, N;Blichfeldt-Eckhardt, M;Koch, S;Deb-Chatterji, M;Magnus, T;Stubbe, J;Madsen, K;Meyer, M;Degn, M;Eisel, ULM;Wlodarczyk, A;Fumagalli, M;Clausen, BH;Brambilla, R;Lambertsen, KL; Microglial TNFR2 signaling regulates the inflammatory response after CNS injury in a sex-specific fashion Brain, behavior, and immunity 2023-12-22 [PMID: 38142915] (IHC, Mouse)
• MC Lund, DG Ellman, M Nissen, PS Nielsen, PV Nielsen, C Jørgensen, DC Andersen, H Gao, R Brambilla, M Degn, BH Clausen, KL Lambertsen The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study Biology, 2022-06-20;11(6):. 2022-06-20 [PMID: 35741460] (ICC, IHC, Mouse)
• Hyehun Choi, Ryan J. Stark, Benjamin S. Raja, Anna Dikalova, Fred S. Lamb Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNF alpha receptor endocytosis American Journal of Physiology-Heart and Circulatory Physiology 2019-06-01 [PMID: 30925081]
• R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Sci Rep, 2017-07-26;7(1):6607. 2017-07-26 [PMID: 28747780] (Mouse)
• S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation Sci Rep, 2017-03-13;7(0):44189. 2017-03-13 [PMID: 28287124] (Flow Cytometry, Mouse)
• Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy Oncotarget, 2016-10-18;0(0):. 2016-10-18 [PMID: 27626702] (Flow Cytometry, Mouse)
• Denise Becker, Thomas Deller, Andreas Vlachos Tumor necrosis factor (TNF)-receptor 1 and 2 mediate homeostatic synaptic plasticity of denervated mouse dentate granule cells Scientific Reports 2015-08-06 [PMID: 26246237]
• Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients. J Immunol, 2014-07-16;193(4):2024-33. 2014-07-16 [PMID: 25031460] (Flow Cytometry, Mouse)
• Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis. PLoS ONE, 2014-02-10;9(2):e88369. 2014-02-10 [PMID: 24520376] (Western Blot, Mouse)
• Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. J. Exp. Med., 2012-05-21;209(6):1219-34. 2012-05-21 [PMID: 22615129] (IHC, Mouse)
• Christopher A. McPherson, Andrew D. Kraft, G. Jean Harry Injury-Induced Neurogenesis: Consideration of Resident Microglia as Supportive of Neural Progenitor Cells Neurotoxicity Research 2011-02-01 [PMID: 20524106] (Immunohistochemistry, Mouse)
• Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest., 2009-09-14;119(10):2954-64. 2009-09-14 [PMID: 19759514] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: TNFRSF1B
• Uniprot:
  • P25119()