TNF RI/TNFRSF1A Antibody (55R170) [Alexa Fluor™ Plus 488]

• Reactivity: Mu
• Clone: 55R170
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Alexa Fluor Plus 488

TNF RI/TNFRSF1A Antibody (55R170) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
  (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody (55R170) [Alexa Fluor™ Plus 488]

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
4. Banner, D.W et al. (1993) Cell 73: 431.
5. Chan, F.K. et al. (2000) Science 288:2351.
6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
7. Jin, L. et al. (2000) J. Immunol. 165:5153.
8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
9. Legler, D.F. et al. (2003) Immunity 18:655.
10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
13. McDermott, M.F. et al. (1999) Cell 97:133.
14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
15. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.