Human TMPRSS2 ELISA Kit (Chemiluminescence)

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Human TMPRSS2 ELISA Kit (Chemiluminescence) [NBP2-89171] - Reference standard curve for Human TMPRSS2 ELISA Kit (Chemiluminescence). These standard curves are provided for demonstration only. A standard curve should be ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

Order Details

Human TMPRSS2 ELISA Kit (Chemiluminescence) Summary

Specificity
This Human TMPRSS2 ELISA Kit (Chemiluminescence)recognizes Human TMPRSS2 in samples. No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
389
Intra-Assay
391
Spike Recovery
87-112%
Sample Volume
100 ul
Kit Type
ELISA Kit (Chemiluminescence)
Gene
TMPRSS2

Applications/Dilutions

Theoretical MW
53.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Product Description
  2. Plate Sealer
  3. Reference Standard
  4. Reference Standard & Sample Diluent
  5. Micro ELISA Plate(Dismountable)
  6. HRP Conjugate Diluent
  7. Concentrated HRP Conjugate (100x)
  8. Concentrated Wash Buffer (25x)
  9. Certificate of Analysis
  10. Concentrated Biotinylated Detection Ab (100x)
  11. Biotinylated Detection Ab Diluent
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human TMPRSS2 ELISA Kit (Chemiluminescence)

  • EC 3.4.21
  • Epitheliasin
  • FLJ41954
  • NP_001128571.1
  • NP_001369649.1
  • NP_005647.3
  • PP9284
  • PRSS10
  • Serine protease 10
  • TMPRSS2
  • transmembrane protease serine 2
  • transmembrane protease, serine 2

Background

TMPRSS2, also called Epitheliasin in mice, is a 492 amino acid type II transmembrane serine protease located on human chromosome 21q22.3 that encodes at least 2 isoforms. This glycosylated serine protease (theoretical molecular weight 70kDa) is regulated by androgens and expressed on the plasma membrane of human bronchial epithelial cells, nasal goblet cells, small intestine epithelia, gastrointestinal tract, the stomach, kidneys and pancreas, with the greatest abundance found in the prostate gland. While the physiological function of TMPRSS2 remains unknown, the serine protease domain undergoes autocleavage and the 32 kDa domain is secreted enabling its interaction with the extracellular matrix. Fusions between TMPRSS2 and the ETS transcription factor genes ERG, ETV1, and ETV4 have been reported in prostate cancer with the TMPRSS2 ERG gene fusion resulting in ERG overexpression in 40-80% of these cases and often producing a more aggressive phenotype. Androgen signaling is disrupted in prostate cancers with the TMPRSS2 ERG fusion which contributes to the switch from androgen dependent to androgen independent prostate cancer (1,2).

TMPRSS2 has also been shown to play a critical role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 at the cell surface to facilitate viral entry. TMPRSS2 mediates viral entry in a similar mechanism for other coronaviruses such as SARS-CoV and MERS. The broad-spectrum serine protease inhibitor, Camostat, is a TMPRSS2 inhibitor demonstrated to protect mice with lethal SARS-CoV infections (3).

References

1. Clark, J., Cooper, C. (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6, 429-439. PMID: 19657377

2. Duffy, MJ. (2014) Chapter One - PSA in Screening for Prostate Cancer: More Good than Harm or More Harm than Good? Adv Clin Chem. 66:1-23. PMID: 25344984

3. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. (2017) TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 142:1-10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for TMPRSS2 ELISA Kit (NBP2-89171). (Showing 1 - 1 of 1 FAQ).

  1. What part of TMPRSS2 does this kit detect?
    • The recombinant extracellular domain of human TMPRSS2 (106-492aa) was applied as the antigen for the antibodies used in this kit. And the standard is also recombinant human TMPRSS2 106-492aa protein. Therefore, this kit is specific to the extracellular domain.

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Blogs on TMPRSS2.

Blocking SARS-CoV-2 Cell Entry: A potential Strategy Against COVID-19 Pandemic
By Jamshed Arslan, Pharm. D., PhD. Coronaviruses are a family of enveloped RNA viruses. Some family members circulate in human populations, but others like severe acute respiratory syndrome coronavirus (SARS-CoV) ar...  Read full blog post.

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Bioinformatics

Gene Symbol TMPRSS2