Human TMPRSS2 ELISA Kit (Chemiluminescence)

ELISA: Human TMPRSS2 ELISA Kit (Chemiluminescence) [NBP2-89171] - Samples were spiked with high concentrations of Human TMPRSS2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human TMPRSS2 ELISA Kit (Chemiluminescence) [NBP2-89171] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human TMPRSS2 ELISA Kit (Chemiluminescence) Summary

• Specificity: This Human TMPRSS2 ELISA Kit (Chemiluminescence)recognizes Human TMPRSS2 in samples. No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 389
• Intra-Assay: CV% < 391
• Spike Recovery: 87-112%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: TMPRSS2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human TMPRSS2 ELISA Kit (Chemiluminescence)

• EC 3.4.21
• Epitheliasin
• FLJ41954
• NP_001128571.1
• NP_001369649.1
• NP_005647.3
• PP9284
• PRSS10
• Serine protease 10
• TMPRSS2
• transmembrane protease serine 2
• transmembrane protease, serine 2

Background

TMPRSS2, also called Epitheliasin in mice, is a 492 amino acid type II transmembrane serine protease located on human chromosome 21q22.3 that encodes at least 2 isoforms. This glycosylated serine protease (theoretical molecular weight 70kDa) is regulated by androgens and expressed on the plasma membrane of human bronchial epithelial cells, nasal goblet cells, small intestine epithelia, gastrointestinal tract, the stomach, kidneys and pancreas, with the greatest abundance found in the prostate gland. While the physiological function of TMPRSS2 remains unknown, the serine protease domain undergoes autocleavage and the 32 kDa domain is secreted enabling its interaction with the extracellular matrix. Fusions between TMPRSS2 and the ETS transcription factor genes ERG, ETV1, and ETV4 have been reported in prostate cancer with the TMPRSS2 ERG gene fusion resulting in ERG overexpression in 40-80% of these cases and often producing a more aggressive phenotype. Androgen signaling is disrupted in prostate cancers with the TMPRSS2 ERG fusion which contributes to the switch from androgen dependent to androgen independent prostate cancer (1,2).

TMPRSS2 has also been shown to play a critical role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 at the cell surface to facilitate viral entry. TMPRSS2 mediates viral entry in a similar mechanism for other coronaviruses such as SARS-CoV and MERS. The broad-spectrum serine protease inhibitor, Camostat, is a TMPRSS2 inhibitor demonstrated to protect mice with lethal SARS-CoV infections (3).

References

1. Clark, J., Cooper, C. (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6, 429-439. PMID: 19657377

2. Duffy, MJ. (2014) Chapter One - PSA in Screening for Prostate Cancer: More Good than Harm or More Harm than Good? Adv Clin Chem. 66:1-23. PMID: 25344984

3. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. (2017) TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 142:1-10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for TMPRSS2 ELISA Kit (NBP2-89171)(2)

Publications using NBP2-89171

• Michael James Houghton, Eglantine Balland, Matthew James Gartner, Belinda Jane Thomas, Kanta Subbarao, Gary Williamson The flavonoid quercetin decreases ACE2 and TMPRSS2 expression but not SARS-CoV-2 infection in cultured human lung cells. BioFactors (Oxford, England) 2024-06-17 [PMID: 38886986]
• Cleemput J, Snippenberg W, Lambrechts L et al. Organ-specific genome diversity of replication-competent SARS-CoV-2 Nat Commun 2021-11-17 [PMID: 34785663] (ELISA)

FAQs for TMPRSS2 ELISA Kit (NBP2-89171). (Showing 1 - 1 of 1 FAQs).

1. What part of TMPRSS2 does this kit detect?
   • The recombinant extracellular domain of human TMPRSS2 (106-492aa) was applied as the antigen for the antibodies used in this kit. And the standard is also recombinant human TMPRSS2 106-492aa protein. Therefore, this kit is specific to the extracellular domain.

Bioinformatics

• Gene Symbol: TMPRSS2