TMEM166 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TMEM166 Antibody [NBP1-92517] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.

Immunohistochemistry-Paraffin: TMEM166 Antibody [NBP1-92517] - Staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.

Staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TMEM166 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TMEM166 Antibody - BSA Free (NBP1-92517) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-TMEM166 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ISCHTDCRRRPGKKFLQDRESSSDSSDSEDGSEDTVSDLSVRRHRRFERTLNKNVFTSAEELERAQRLEERERIIREIWMNGQPEVPGTRSLNRYY
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EVA1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TMEM166 Protein (NBP1-92517PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TMEM166 Antibody - BSA Free

• family with sequence similarity 176, member A
• TMEM166
• Transmembrane protein 166FLJ13391

Background

FUNCTION: Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Lysosome membrane; Single-pass membrane protein. TISSUE SPECIFICITY: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM166 Antibody (NBP1-92517)(1)

Publication using NBP1-92517

• Liu B, Zhou Y, Wu Q et al. EVA1A regulates hematopoietic stem cell regeneration via ER-mitochondria mediated apoptosis Cell Death & Disease 2023-01-30 [PMID: 36717548]

Bioinformatics

• Gene Symbol: EVA1A