TMEM119 Antibody (1023426) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with human TMEM119 and eGFP was stained with (A) Mouse anti-human TMEM119 Alexa Fluor® 647-conjugated monoclonal antibody (Catalog # FAB10313R) or (B) Mouse IgG1 isotype control antibody (IC002R). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 1023426
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

TMEM119 Antibody (1023426) [Alexa Fluor® 647] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO derived human TREM119
  Arg26-Met96
  Accession # Q4V9L6.1
• Specificity: Detects human TREM119 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMEM119 Antibody (1023426) [Alexa Fluor® 647]

• OBIF
• Osteoblast Induction Factor
• TMEM119

Background

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3). Mature human TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 166 aa cytoplasmic domain. Within the ECD, human TMEM119 shares 78% and 75% aa sequence identity with mouse and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).

1. Jiang, Z,H. et al. (2017) Expt & Mol Med. 49:e329.
2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
3. Satoh, J. et al. (2016) Neuropathol. 36:39.
4. Kanamoto, T. et al. (2009) BMC Develop. Biol. 9:70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q4V9L6.1()