TMEM100 Antibody (OTI10G3)

Western Blot: TMEM100 Antibody (10G3) [NBP1-48024] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100.

Immunocytochemistry/Immunofluorescence: TMEM100 Antibody (10G3) [NBP1-48024] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM100.

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human pancreas tissue using anti-TMEM100 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human colon tissue using anti-TMEM100 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human endometrium tissue using anti-TMEM100 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human Kidney tissue using anti-TMEM100 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TMEM100 Antibody (10G3) [NBP1-48024] - Staining of paraffin-embedded Human Ovary tissue using anti-TMEM100 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI10G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.4 mg/ml

TMEM100 Antibody (OTI10G3) Summary

• Immunogen: Full length human recombinant protein of human TMEM100 (NP_060756) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens transmembrane protein 100 (TMEM100), transcript variant 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEM100
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 14.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMEM100 Antibody (OTI10G3)

• FLJ10970
• FLJ37856
• transmembrane protein 100

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEM100
• Entrez:
  • 55273(Human)