Recombinant Human BMP-10 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-10 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 15-60 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-10 protein
Asn317-Arg424
Accession #
N-terminal Sequence
Asn317
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP10
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
2926-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-10 Protein

  • BMP10
  • BMP-10
  • bone morphogenetic protein 10
  • MGC126783

Background

BMP-10, along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1-3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked
non-glycosylated homodimers (4, 5). Mature human BMP-10 shares 98% amino acid sequence identity with mouse and rat BMP-10 and 49% - 63% with human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart but is not expressed until after cardiac patterning or looping are completed (6-8). BMP-10 production appears at the onset of trabeculation and chamber formation and is restricted to the right atrium in the adult heart (6-8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for the expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and the growth of embryonic cardiomyocytes (7, 10). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 over-expression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to signal through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA causes similar cardiac morphogenetic abnormalities (11). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
  4. Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
  5. David, L. et al. (2007) Blood 109:1953.
  6. Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
  7. Chen, H. et al. (2004) Development 131:2219.
  8. Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
  9. Srivastava, D. and E.N. Olson (2000) Nature 407:221.
  10. Pashmforoush, M. et al. (2004) Cell 117:373.
  11. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.

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Publications for BMP-10 (2926-BP)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, complex formation.


Filter By Application
Bioassay
(7)
Surface Plasmon Resonance
(1)
complex formation
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 2926-BP Applications Species
CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2 J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28646109] (Bioassay, Human) Bioassay Human
Y Tachida, N Izumi, T Sakurai, H Kobayashi Mutual interaction between endothelial cells and mural cells enhances BMP9 signaling in endothelial cells Biol Open, 2017;6(3):370-380. 2017 [PMID: 28298363] (Bioassay, Human) Bioassay Human
Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus. Proc Natl Acad Sci U S A, 2015;112(25):E3207-15. 2015 [PMID: 26056270] (Bioassay, Human) Bioassay Human
Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE, 2015;10(4):e0125697. 2015 [PMID: 25919028] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling. Blood, 2012;119(25):6162-71. 2012 [PMID: 22566602] (Bioassay, Human) Bioassay Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Herrera B, Inman GJ, A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol., 2009;10(0):20. 2009 [PMID: 19298647] (Bioassay, Mouse) Bioassay Mouse
Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008;283(20):13874-88. 2008 [PMID: 18339631] (complex formation, Human) complex formation Human

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Bioinformatics

Gene Symbol BMP10
Entrez
Uniprot