Hsp47 Antibody (M16.10A1)

Images

 
Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 ...read more
Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.
Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO ...read more
Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb ...read more
Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, ShSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Clone
M16.10A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Hsp47 Antibody (M16.10A1) Summary

Immunogen
Native rat HSP47.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SERPINH1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
Application Notes
This antibody is useful in IHC and detects a band of ~47kDa by WB. Immunoprecipitation, Flow Cytometry and ICC/IF were reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-97491 in the following applications:

Publications
Read Publications using
NBP1-97491 in the following applications:

  • 1 publication
  • 3 publications
  • IHC
    4 publications
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Recognizes Sheep HSP47. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Hsp47 Antibody (M16.10A1)

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hsp47 Antibody (NBP1-97491)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IP, WB.


Filter By Application
FLOW
(1)
ICC/IF
(3)
IHC
(4)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
Rat
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-97491 Applications Species
Khalil H, Kanisicak O, Vagnozzi RJ et al. Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart JCI Insight Aug 8 2019 [PMID: 31393098] (WB, Mouse) WB Mouse
Chu, SY;Chou, CH;Huang, HD;Yen, MH;Hong, HC;Chao, PH;Wang, YH;Chen, PY;Nian, SX;Chen, YR;Liou, LY;Liu, YC;Chen, HM;Lin, FM;Chang, YT;Chen, CC;Lee, OK; Mechanical stretch induces hair regeneration through the alternative activation of macrophages Nat Commun Apr 3 2019 [PMID: 30944305] (ICC/IF, Mouse) ICC/IF Mouse
A Furusu et al. Renoprotective Effect of Azelnidipine in Rats. Biol. Pharm. Bull. 31, 2237. 2008 [PMID: 19043206] (IHC, Rat) IHC Rat
D Li et al. Novel Adenoviral Gene Delivery System Targeted Against Head and NeckCancer. Laryngoscope 118, 650. 2008 [PMID: 18176343] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Y Ogawa et al. Role of heat shock protein 47, a collagen-binding chaperone, inlacrimal gland pathology in patients with cGVHD. Invest. Ophthalmol. Vis. Sci. 48, 1079. 2007 [PMID: 17325149] (IHC, ICC/IF, WB, Human) IHC, ICC/IF, WB Human
L Wogensen et al. Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta1-induced renal interstitial fibrosis. Histochem. Cell Biol. 119, 267. 2003 [PMID: 12684813] (IHC, WB, Mouse) IHC, WB Mouse
K Nagata et al. Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease. Biochem. Biophys. Res. Commun. 303, 413. 2003 [PMID: 12659832] (Human) Human
JG Pickering et al. Functional Linkage between the Endoplasmic Reticulum Protein Hsp47 and Procollagen Expression in Human Vascular Smooth Muscle Cells. J. Biol. Chem. 277, 38571. 2002 [PMID: 12163502] (WB, Human) WB Human
NJ Bulleid et al. Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47. J. Biol. Chem. 277, 35007. 2002 [PMID: 12114508] (IP, Human) IP Human
JG Pickering et al. HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen. Arterioscler. Thromb. Vasc. Biol. 21, 40. 2001 [PMID: 11145931] (WB, Rat) WB Rat
Show All 11 Publications.

Reviews for Hsp47 Antibody (NBP1-97491) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NBP1-97491:
Filter by Applications
WB
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Hsp47 NBP1-97491
Enlarge
reviewed by:
Shenda Gu
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedNIH3T3 Whole Cell Lysate, 20ug loaded per lane
SpeciesMouse

Blocking

Blocking DetailsLicor Odyssey Blocking Buffer, 1 hr at room temperature

Primary Anitbody

Dilution Ratio1:1000 in Licor Odyssey Blocking Buffer, overnight at 4 degrees

Secondary Antibody

Secondary DescriptionDonkey anti-mouse IRDye 800CW
Secondary Manufacturer Cat#926-32212
Secondary Concentration1:10,000

Details

Detection NotesLicor Odyssey Scanner, sensitivity set to 6

Comments

Comments5x10^4 NIH3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection.
Immunohistochemistry-Frozen Hsp47 NBP1-97491
Enlarge
reviewed by:
Anonymous
IHC-Fr Other 03/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedGuinea pig skin
SpeciesOther

Primary Anitbody

Dilution Ratio1:100 at 4 degrees C overnight

Secondary Antibody

Secondary Descriptiondonkey anti-mouse Alexa Fluor 555
Secondary Concentration1:500

Details

Fixation Details4% PFA
Wash Description1xPBS 3x 5min each

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Hsp47 Antibody (NBP1-97491) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Shenda Gu
01/07/2015
Application: WB
Species: Mouse

Anonymous
03/28/2014
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol SERPINH1