TLK1 Antibody - BSA Free

Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Western blot using Affinity Purified antibody shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1. Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TLK1 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human Tlk-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified TLK1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human TLK1 (Tousled-like kinase) (isoform 1) protein. (Uniprot: Q9UKI8)
• Specificity: This affinity purified antibody is directed against human TLK1 protein. This antibody reacts with both phosphorylated and non-phosphorylated forms of TLK1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of TLK1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with TLK1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with TLK1 homologues from other sources has not been determined.
• Predicted Species: Mouse (96%), Rat (93%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Western Blot 1:200-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with TLK1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with TLK1 homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TLK1 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• KIAA0137serine/threonine-protein kinase tousled-like 1
• PKU-beta
• SNARE protein kinase SNAK
• tousled-like kinase 1serine threonine protein kinase

Background

Tousled-like kinase 1 (TLK1) is a serine/threonine kinase involved in chromatin assembly and the DNA damage response. The tousled (Tsl) kinase was first identified in Arabidopsis thaliana and speculated to be involved in cell division during patterning of plant organs. There are two tousled-like kinases in humans, TLK1 and TLK2, that can dimerize or heterodimerize for function and can be phosphorylated and inactivated by Chk1 in response to DNA damage. The histone chaperone, Asf1 (anti-silencing function 1), has been reported as a substrate for both TLK1 and TLK2. TLK1 has also been shown to be important for cell cycle progression and proper chromosome segregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLK1
• Uniprot:
  • NP_036422(Human)
  • Q9UKI8(Human)