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ASF1b Antibody (6G7G4) - BSA Free

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Western Blot: ASF1b Antibody (6G7G4) [NBP2-61684] - Analysis using ASF1B mAb against HEK293 (1) and ASF1B (AA: 1-202)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: ASF1b Antibody (6G7G4) [NBP2-61684] - Analysis using ASF1B mAb against HEK293 (1) and ASF1B (AA: 1-202)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: ASF1b Antibody (6G7G4) [NBP2-61684] - Analysis of K562 cells using ASF1B mouse mAb (green) and negative control (red).
Western Blot: ASF1b Antibody (6G7G4) [NBP2-61684] - Analysis using ASF1B mAb against human ASF1B (AA: 1-202) recombinant protein. (Expected MW is 48.4 kDa)
Western Blot: ASF1b Antibody (6G7G4) [NBP2-61684] - Analysis using ASF1B mouse mAb against Hela (1), COS7 (2), HCT116 (3), and CHO3D10 (4) cell lysate.
ELISA: ASF1b Antibody (6G7G4) [NBP2-61684] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow
Clone
6G7G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-61684-0.025ml
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ASF1b Antibody (6G7G4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ASF1B
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
Theoretical MW
22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ASF1b Antibody (6G7G4) - BSA Free

  • ASF1 silencing function 1 homolog B (S. cerevisiae)
  • CCG1-interacting factor A-II
  • CIA-II
  • FLJ10604
  • hAsf1
  • hAsf1b
  • hCIA-II
  • histone chaperone ASF1B
  • silencing function protein 1 homolog B

Background

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: ASF1a Antibody [NBP2-98775] - ASF1a was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 ul anti-ASF1a rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ASF1a rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 35 kDa
Immunocytochemistry/Immunofluorescence: ASF1a Antibody [NBP2-98775] - Immunofluorescence staining of ASF1a in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ASF1a polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.
Western Blot: ASF1a Antibody [NBP2-98775] - Anti-ASF1a rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
KAT4/TBP
Associated Factor 1 A
NBP1-77847
Immunohistochemistry: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-TAF1 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by Chip Kit. CHIP was performed on fos promoters using 5 ug of TAF1 antibody and an RNA PolII antibody.
Western Blot: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.
Species: Hu, Mu
Applications: ChIP, ELISA, IHC, IHC-P, IP, NULL, WB
HIST1H4F
Overexpression Lysate
NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
Species: Hu
Applications: WB
H4/n Antibody
(3E7H2) - BSA Free
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ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).
Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Antibody - BSA Free
NLS3775
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Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma
(1 Publication)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
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NBP1-30141
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
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Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
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(2F6)
H00029883-M01
Immunocytochemistry/Immunofluorescence: CNOT7 Antibody (2F6) [H00029883-M01] - Analysis of monoclonal antibody to CNOT7 on HeLa cell. Antibody concentration 40 ug/ml.
Sandwich ELISA: CNOT7 Antibody (2F6) [H00029883-M01] - Detection limit for recombinant GST tagged CNOT7 is 3 ng/ml as a capture antibody.
Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6 Analysis of CNOT7 expression in HeLa.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
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(SS 53)
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Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.
(8 Publications)
Species: Hu
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TATA binding
protein TBP Antibody
NBP3-13174
Western Blot: TATA binding protein TBP Antibody [NBP3-13174] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications: IHC, IHC-P, WB
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NBP1-83035
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Western Blot: TLK1 Antibody [NBP1-83035] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Western Blot: TLK1 Antibody [NBP1-83035] - Analysis in control (vector only transfected HEK293T lysate) and TLK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
CHAF1A Antibody
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NB500-207
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
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NBP3-04894
Immunohistochemistry-Paraffin: HIRA Antibody [NBP3-04894] - Paraffin-embedded human gastric cancer using HIRA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: HIRA Antibody [NBP3-04894] - Paraffin-embedded human lung cancer using HIRA antibody at dilution of 1:100 (40x lens).
Western Blot: HIRA Antibody [NBP3-04894] - Analysis of extracts of various cell lines, using HIRA antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Species: Hu
Applications: IHC, IHC-P, WB
Host Cell Factor
1/HCFC1 Antibody
NB100-68210
Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY licence.
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IP, Simple Western, WB
Histone H3.1
Antibody
NBP2-75524
Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
HIST1H3C
Overexpression Lysate
NBP2-07993
Western Blot: HIST1H3C Overexpression Lysate (Adult Normal) [NBP2-07993] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H3C.
Species: Hu
Applications: WB
RbAp46 Antibody
(OTI5A4)
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Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.
Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RbAp46 mouse monoclonal antibody.
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Applications: IHC, IHC-P, WB
ASF1b Antibody
(6G7G4) - BSA Free
NBP2-61684
ASF1b Antibody (6G7G4) - BSA Free
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow

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Bioinformatics

Gene Symbol ASF1B