TIN2 Antibody (59B388.1)

Product Discontinued

TIN2 Antibody (59B388.1) Summary

• Immunogen: A synthetic peptide corresponding to amino acids 44-58 of human Tin2 was used as immunogen (NP_036593.2|).
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TINF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Gel Super Shift Assays
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1-3 ug/ml
• Application Notes: Useful in Gel Super Shift Assays see Choi et al. In Western Blot: Detects a band of approximately 40 kDa. Use in ICC/IF reported in scientific literature (PMID 26230315). Use in Immunohistochemistry reported in scientific literature (PMID: 16449311). Use in Gel super shift assay reported in scientific literature (PMID: 21852327). Use in immunoblotting reported in scientific literature (PMID: 23708666).
• Control:

  293 Whole Cell Lysate
  TIN2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TIN2 Antibody (59B388.1)

• (TRF1)-interacting nuclear factor 2 variant 1
• TERF1 (TRF1)-interacting nuclear factor 2
• TERF1-interacting nuclear factor 2
• TIN2TRF1-interacting nuclear protein 2

Background

TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenous TRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIN2 Antibody (NB600-1522)(14)

Publications using NB600-1522

• Yu EY, Zahid SS, Aloe S Et al. Reciprocal impacts of telomerase activity and ADRN/MES differentiation state in neuroblastoma tumor biology Communications biology 2021-11-19 [PMID: 34799676] (WB, Human)
• Vessoni AT, Zhang T, Quinet A et al. Telomere erosion in human pluripotent stem cells leads to ATR-mediated mitotic catastrophe The Journal of cell biology 2021-06-07 [PMID: 33851958]
• Frank AK, Tran DC, Qu RW et al. The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres. PLoS Genet. 2015-07-31 [PMID: 26230315] (ICC/IF, WB, Mouse)
• Saha Bidisha, Zitnik Galynn, Johnson Simon et al. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations. Front Genet. 2013-01-01 [PMID: 23847654] (WB)
• Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene. 2013-05-27 [PMID: 23708666]
• Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res. 2011-11-01 [PMID: 21852327] (GS)
  
  Details:
  Tin2 (IMG-282) & POT1 (IMG-5343A). EMSA: HeLa cell line transfected with or without Flag-Tin2, Fig 4C.
• Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011-06-01 [PMID: 21394098] (WB)
  
  Details:
  WB: TRF2 (IMG-124), Tankyrase (IMG-146), POT1 (IMG-5343A), and Tin2 (IMG-282). EBNA1 positive or negative Ramos and BJAB cell lines, Fig 4A.
• Cesare AJ, Kaul Z, Cohen SB et al. Spontaneous occurrence of telomeric DNA damage response in the absence of chromosome fusions. Nat Struct Mol Biol. 2009-12-01 [PMID: 19935685] (WB, IF)
• Jiang WQ, Zhong ZH, Henson JD et al. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene. 2007-07-12 [PMID: 17297460] (WB)
  
  Details:
  Tin/Tin2 (clone 59B288.1, IMG-282) and TRF2 (clone 4A794.15, IMG-124A) in IIICF/c human Li-Fraumeni syndrome spontaneously immortalized fibroblast cells treated with TIN2, TRF2 or control siRNA: WB (Fig 4a), IF (Figs 4d and 5). Additionally, the TRF2 mAb was also used for IF in Figs 3C, 6D Notes: The specificity of the Tin2 and TRF2 mAbs were siRNA validated by WB in Fig 4a and by IF in Figs 4d and 5. For IF, cells cultured in chamber slides were fixed for 15 min in 2% parafomaldehyde at room temperature and then permeabilized with methanol/acetone (1:1) at 20 degrees C for 15 min. They were incubated with the Tin2 or TRF2 mAb overnight at 4 degrees and then with secondary antibodies for 40 min at room temperature. In some cases, DAPI was included with the secondary antibody to visualize DNA.
• Roig I, Garcia R, Robles P et al. Human fetal ovarian culture permits meiotic progression and chromosome pairing process. Hum Reprod. 2006-06-01 [PMID: 16449311] (IF/IHC)
• Roig I, Robles P, Garcia R et al. Chromosome 18 pairing behavior in human trisomic oocytes. Presence of an extra chromosome extends bouquet stage. Reproduction. 2005-05-01 [PMID: 15855620] (ICC/IF, Human)
  
  Details:
  IF/ICC (human telomeres), Fig 1.
• Houghtaling BR, Cuttonaro L, Chang W, Smith S. A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2. Curr Biol. 2004-09-21 [PMID: 15380063] (IF)
• Kim SH, Beausejour C, Davalos AR et al. TIN2 mediates functions of TRF2 at human telomeres. J Biol Chem. 2004-10-15 [PMID: 15292264]
• Roig I, Liebe B, Egozcue J et al. Female-specific features of recombinational double-stranded DNA repair in relation to synapsis and telomere dynamics in human oocytes. Chromosoma. 2004-08-01 [PMID: 15235794] (ICC/IF)

Review for TIN2 Antibody (NB600-1522) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/05/2019

Summary

• Application: Western Blot
• Sample Tested: Primary CD4+ T-cells isolated from peripheral blood.
• Species: Human
• Lot: AB071012B-02

Bioinformatics

• Gene Symbol: TINF2
• Entrez:
  • 26277(Human)
• Uniprot:
  • Q9BSI4(Human)