TIN2 Antibody (59B388.1)

Images

 
Western Blot: TIN2 Antibody (59B388.1) [NB600-1522] - CD4+ T-cells were isolated from HS PBMCs after 2 days incubation with or without 5 uM KML001. Image from verified customer review.
Western Blot: TIN2 Antibody (59B388.1) [NB600-1522] - Analysis of Tin2 using Tin2 monoclonal antibody 59B388.1. Human Daudi (A) and mouse NIH 3T3 lysate probed using Tin2 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, GS, IB, ICC/IF, IHC
Clone
59B388.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TIN2 Antibody (59B388.1) Summary

Immunogen
A synthetic peptide corresponding to amino acids 44-58 of human Tin2 was used as immunogen (NP_036593.2|).
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TINF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Gel Super Shift Assays
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Useful in Gel Super Shift Assays see Choi et al. In Western Blot: Detects a band of approximately 40 kDa. Use in ICC/IF reported in scientific literature (PMID 26230315). Use in Immunohistochemistry reported in scientific literature (PMID: 16449311). Use in Gel super shift assay reported in scientific literature (PMID: 21852327). Use in immunoblotting reported in scientific literature (PMID: 23708666).
Control
293 Whole Cell Lysate
TIN2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-1522 in the following applications:

Publications
Read Publications using
NB600-1522 in the following applications:

  • GS
    1 publication
  • 3 publications
  • IF
    2 publications
  • IHC
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TIN2 Antibody (59B388.1)

  • (TRF1)-interacting nuclear factor 2 variant 1
  • TERF1 (TRF1)-interacting nuclear factor 2
  • TERF1-interacting nuclear factor 2
  • TIN2TRF1-interacting nuclear protein 2

Background

TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenous TRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TIN2 Antibody (NB600-1522)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: GS, ICC/IF, IF, IHC, WB.


Filter By Application
GS
(1)
ICC/IF
(3)
IF
(2)
IHC
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-1522 Applications Species
Frank AK, Tran DC, Qu RW et al. The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres. PLoS Genet. 2015 Jul 01 [PMID: 26230315] (WB, ICC/IF, Mouse)

Details:
TIN2 antibody was used for ICC/IF assay coupled with FISH analysis (a PNA telomeric probe) in cells carrying heterozygous TIN2 mutation (clone R282H.1, PD8 cells) and wild-type TIN2 (clone WT.1, PD8 cells) [Fig 1]. The antibody was also used for WB on nuclear extracts of the HCT116 knock-in clones, HCT116 parental cells, and TIN2 shRNA-treated HCT116 cells. This antibody is developed against an N-terminal AA 44–58 of TIN2 and recognizes both the wild-type as well as mutant TIN2 (Fig S2).
WB, ICC/IF Mouse
Saha Bidisha, Zitnik Galynn, Johnson Simon et al. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations. Front Genet. 2013 [PMID: 23847654] (WB) WB
Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene. 2013 May 27 [PMID: 23708666]
Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res. 2011 Nov. [PMID: 21852327] (GS)

Details:
Tin2 (IMG-282) & POT1 (IMG-5343A). EMSA: HeLa cell line transfected with or without Flag-Tin2, Fig 4C.
GS
Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011 Jun [PMID: 21394098] (WB)

Details:
WB: TRF2 (IMG-124), Tankyrase (IMG-146), POT1 (IMG-5343A), and Tin2 (IMG-282). EBNA1 positive or negative Ramos and BJAB cell lines, Fig 4A.
WB
Cesare AJ, Kaul Z, Cohen SB et al. Spontaneous occurrence of telomeric DNA damage response in the absence of chromosome fusions. Nat Struct Mol Biol. 2009 Dec [PMID: 19935685] (WB, IF)

Details:
mAbs cited: 1. Tin/Tin2 (clone 59B288.1, IMG-282): IF (Fig 6D), GM847 cells 2. TRF2 (clone 4A794.15, IMG-124A): WB (Fig 5C) and IF (Figs 5C, 5F), TRF2 retroviral transduced human cell lines (GM847, Saos-2, and IIICF/c) IF (Fig 6D), GM847 cells WB (Fig S9A
WB, IF
Jiang WQ, Zhong ZH, Henson JD et al. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene. 2007 Jul 12 [PMID: 17297460] (WB)

Details:
Tin/Tin2 (clone 59B288.1, IMG-282) and TRF2 (clone 4A794.15, IMG-124A) in IIICF/c human Li-Fraumeni syndrome spontaneously immortalized fibroblast cells treated with TIN2, TRF2 or control siRNA: WB (Fig 4a), IF (Figs 4d and 5). Additionally, the TRF2 mAb was also used for IF in Figs 3C, 6D Notes: The specificity of the Tin2 and TRF2 mAbs were siRNA validated by WB in Fig 4a and by IF in Figs 4d and 5. For IF, cells cultured in chamber slides were fixed for 15 min in 2% parafomaldehyde at room temperature and then permeabilized with methanol/acetone (1:1) at 20 degrees C for 15 min. They were incubated with the Tin2 or TRF2 mAb overnight at 4 degrees and then with secondary antibodies for 40 min at room temperature. In some cases, DAPI was included with the secondary antibody to visualize DNA.
WB
Roig I, Garcia R, Robles P et al. Human fetal ovarian culture permits meiotic progression and chromosome pairing process. Hum Reprod. 2006 Jun [PMID: 16449311] (IHC) IHC
Roig I, Robles P, Garcia R et al. Chromosome 18 pairing behavior in human trisomic oocytes. Presence of an extra chromosome extends bouquet stage. Reproduction. 2005 May [PMID: 15855620] (ICC/IF, Human)

Details:
IF/ICC (human telomeres), Fig 1.
ICC/IF Human
Houghtaling BR, Cuttonaro L, Chang W, Smith S. A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2. Curr Biol. 2004 Sep 21 [PMID: 15380063] (IF)

Details:
1. Tin/Tin2 (clone 59B288.1, IMG-282): IF, Fig 2 (HeLal.2.11 cells) and Fig 6B (Tankyrase1 overexpressing cells)
IF
Show All 12 Publications.

Review for TIN2 Antibody (NB600-1522) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1522:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TIN2 NB600-1522
Enlarge
reviewed by:
Anonymous
WB Human 06/05/2019
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Summary

ApplicationWestern Blot
Sample TestedPrimary CD4+ T-cells isolated from peripheral blood.
SpeciesHuman
LotAB071012B-02

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TIN2 Antibody (NB600-1522) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1522AF350
Alexa Fluor 405 NB600-1522AF405
Alexa Fluor 488 NB600-1522AF488
Alexa Fluor 532 NB600-1522AF532
Alexa Fluor 594 NB600-1522AF594
Alexa Fluor 647 NB600-1522AF647
Alexa Fluor 700 NB600-1522AF700
Alexa Fluor 750 NB600-1522AF750
Biotin NB600-1522B
DyLight 350 NB600-1522UV
DyLight 405 NB600-1522V
DyLight 488 NB600-1522G
DyLight 550 NB600-1522R
DyLight 594 NB600-1522DL594
DyLight 650 NB600-1522C
DyLight 680 NB600-1522FR
DyLight 755 NB600-1522IR
FITC NB600-1522F
HRP NB600-1522H
Janelia Fluor 549 NB600-1522JF549
Janelia Fluor 646 NB600-1522JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/05/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol TINF2
Entrez
Uniprot