TIN2 Antibody (59B388.1)


Western Blot: TIN2 Antibody (59B388.1) [NB600-1522] - Analysis of Tin2 using Tin2 monoclonal antibody 59B388.1. Human Daudi (A) and mouse NIH 3T3 lysate probed using Tin2 antibody at 2 ug/ml.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, GS, ICC/IF
0.5 mg/ml

TIN2 Antibody (59B388.1) Summary

A synthetic peptide corresponding to amino acids 44-58 of human Tin2 was used as immunogen (NP_036593.2|).
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS with 0.05% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


  • Western Blot 1-3 ug/ml
  • Gel Super Shift Assays 1:1-1:100
  • Immunocytochemistry/Immunofluorescence
Application Notes
Useful in Gel Super Shift Assays see Choi et al. In Western Blot: Detects a band of approximately 40 kDa. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26230315)
Read Publications using
NB600-1522 in the following applications:

  • GS
    1 publication
  • 3 publications
  • IF
    2 publications
  • IHC
    1 publication
  • WB
    5 publications

Alternate Names for TIN2 Antibody (59B388.1)

  • (TRF1)-interacting nuclear factor 2 variant 1
  • TERF1 (TRF1)-interacting nuclear factor 2
  • TERF1-interacting nuclear factor 2
  • TIN2TRF1-interacting nuclear protein 2


TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenous TRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, IP
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Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IHC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, GS, ICC/IF
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Publications for TIN2 Antibody (NB600-1522)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: GS, ICC/IF, IF, IHC, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-1522 Applications Species
Frank AK, Tran DC, Qu RW et al. The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres. PLoS Genet. 2015 Jul 01 [PMID:26230315] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Saha Bidisha, Zitnik Galynn, Johnson Simon et al. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations. Front Genet. 2013 [PMID:23847654] (WB) WB
Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene. 2013 May 27 [PMID:23708666]
Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res. 2011 Nov. [PMID:21852327] (GS) GS
Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011 Jun [PMID:21394098] (WB) WB
Cesare AJ, Kaul Z, Cohen SB et al. Spontaneous occurrence of telomeric DNA damage response in the absence of chromosome fusions. Nat Struct Mol Biol. 2009 Dec [PMID:19935685] (WB, IF) WB, IF
Jiang WQ, Zhong ZH, Henson JD et al. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene. 2007 Jul 12 [PMID:17297460] (WB) WB
Roig I, Garcia R, Robles P et al. Human fetal ovarian culture permits meiotic progression and chromosome pairing process. Hum Reprod. 2006 Jun [PMID:16449311] (IHC) IHC
Roig I, Robles P, Garcia R et al. Chromosome 18 pairing behavior in human trisomic oocytes. Presence of an extra chromosome extends bouquet stage. Reproduction. 2005 May [PMID:15855620] (ICC/IF, Human) ICC/IF Human
Houghtaling BR, Cuttonaro L, Chang W, Smith S. A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2. Curr Biol. 2004 Sep 21 [PMID:15380063] (IF) IF
Show All 12 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TIN2 Antibody (NB600-1522) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB600-1522AF405
Alexa Fluor (R) 488 Labeled NB600-1522AF488
Alexa Fluor (R) 647 Labeled NB600-1522AF647
Alexa Fluor (R) 700 Labeled NB600-1522AF700
Allophycocyanin Labeled NB600-1522APC
Biotin Labeled NB600-1522B
DyLight 350 Labeled NB600-1522UV
DyLight 405 Labeled NB600-1522V
DyLight 405LS Labeled NB600-1522V3
DyLight 488 Labeled NB600-1522G
DyLight 550 Labeled NB600-1522R
DyLight 650 Labeled NB600-1522C
DyLight 680 Labeled NB600-1522FR
DyLight 755 Labeled NB600-1522IR
HRP Labeled NB600-1522H
PE Labeled NB600-1522PE
PerCP Labeled NB600-1522PCP

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Blogs on TIN2

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Contact Information

Product PDFs


Gene Symbol TINF2

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