TIMP-2 Antibody (3A4)

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: 3A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TIMP-2 Antibody (3A4) Summary

• Description: For a positive control use Colon carcinoma.
• Immunogen: Human TIMP-2 peptide: DSGNDIYGNPI
• Specificity: Does not cross-react with TIMP-1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Hybridoma culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

For a positive control use Colon carcinoma.

Alternate Names for TIMP-2 Antibody (3A4)

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

TIMP-2 (Tissue inhibitor of metalloproteinase 2) is a nonglycosylated protein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD. The mechanisms of TIMPs inhibit angiogenesis appear to be specific for each member of the TIMP family. TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin alpha3 beta1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. Clone F27P3A4 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human, mouse, rabbit, chicken, zebrafish, and pig TIMP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-2 Antibody (NBP1-42375)(3)

Publications using NBP1-42375

• Chen G, Ge D, Zhu B et al. Salvia miltiorrhiza Injection Alleviates LPS-Induced Acute Lung Injury by Adjusting the Balance of MMPs/TIMPs Ratio Evidence-Based Complementary and Alternative Medicine 2020-07-20 [PMID: 32765635] (WB, Rat)
• Chen G, Ge D, Zhu B et al. Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury J. Int. Med. Res. 2020-04-01 [PMID: 32339071]
• Chuang CH, Yeh CL, Yeh SL et al. Quercetin metabolites inhibit MMP-2 expression in A549 lung cancer cells by PPAR-gamma associated mechanisms. The Journal of Nutritional Biochemistry. 2016-03-31 [PMID: 27260467]

Bioinformatics

• Gene Symbol: TIMP2