TIM-4 Antibody (921832R) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human TIM-4 and eGFP was stained with and either (A) Mouse Anti-Human TIM-4 Monoclonal Antibody (Catalog # MAB2929R) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 921832R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TIM-4 Antibody (921832R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Mouse myeloma cell line NSO-derived recombinant human TIM-4
  Glu25-Leu315
  Accession # Q96H15
• Specificity: Detects human TIM-4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-4 Antibody (921832R) [Unconjugated]

• FLJ27515
• Smuckler
• T-cell immunoglobulin and mucin domain containing 4
• T-cell immunoglobulin and mucin domain-containing protein 4
• T-cell membrane protein 4
• TIM4
• TIM-4
• TIMD4
• TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5-7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4 + T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. 
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96H15()