TIM-3 Antibody (215015)

• Reactivity: Mu
• Applications: WB
• Clone: 215015
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-3 Antibody (215015) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIM‑3
  Arg20-Ala193
  Accession # Q8VIM0
• Specificity: Detects mouse TIM-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) TIM-1, recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (215015)

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9-12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling (10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development (7), inflammation (15), and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).

1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
4. Monney, L. et al. (2002) Nature 415:536.
5. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
6. Geng, H. et al. (2006) J. Immunol. 176:1411.
7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
9. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
10. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
11. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
12. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
13. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
14. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
15. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-3 Antibody (MAB15291)(1)

Publication using MAB15291

• Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood, 2007-07-09;110(7):2565-8. 2007-07-09 [PMID: 17620455] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HAVCR2
• Uniprot:
  • Q8VIM0()