TIM-2 Antibody (RM0134-5B23) [CoraFluor™ 1]

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0134-5B23
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

TIM-2 Antibody (RM0134-5B23) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Mouse recombinant protein of TIM-2
• Specificity: This antibody does not cross-react with mouse TIM-1 and TIM-3.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TIM-2 Antibody (RM0134-5B23) [CoraFluor™ 1]

• G9D3
• T-cell immunoglobulin and mucin domain-containing protein 2
• T-cell immunoglobulin mucin receptor 2
• T-cell membrane protein 2
• TIM2
• TIM-2
• TIMD-2

Background

Tim-2 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-2. It is expressed on activated T cells and preferentially on differentiated Th2 cells. Sema4A is the ligand of Tim-2. A blockade of Tim-2/Tim-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. Tim-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.