TIM-1/KIM-1/HAVCR Antibody (222414) [Unconjugated]

HEK293 cells transfected with Mouse TIM-1 were stained with Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, ELISA(Cap)
• Clone: 222414
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-1/KIM-1/HAVCR Antibody (222414) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
  Tyr22-Thr212
  Accession # NP_001160104
• Specificity: Detects mouse TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. This antibody does not cross-react with recombinant human (rh) TIM‑1, rmTIM-2, or rmTIM-3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Mouse TIM-1/KIM-1/HAVCR Antibody (Catalog # MAB1817)
  ELISA Detection: Mouse TIM-1/KIM-1/HAVCR Biotinylated Antibody (Catalog # BAF1817)
  Standard: Recombinant Mouse TIM-1/KIM-1/HAVCR (Catalog # 1817-TM)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (222414) [Unconjugated]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 or HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39% and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (MAB1817)(26)

Publications using MAB1817

• McLarnon, SR;Honeycutt, SE;N'Guetta, PY;Xiong, Y;Li, X;Abe, K;Kitai, H;Souma, T;O'Brien, LL; Altered renal vascular patterning reduces ischemic kidney injury and limits vascular loss associated with aging bioRxiv : the preprint server for biology 2024-11-01 [PMID: 39553980] (Immunohistochemistry, Transgenic Mouse)
• RGJ Rietjens, G Wang, AIM van der Ve, A Koudijs, MC Avramut, S Kooijman, PCN Rensen, J van der Vl, TJ Rabelink, B Heijs, BM van den Be Phosphatidylinositol metabolism of the renal proximal tubule S3 segment is disturbed in response to diabetes Scientific Reports, 2023-04-17;13(1):6261. 4/17/2023 [PMID: 37069341]
• Gangqi Wang, Bram Heijs, Sarantos Kostidis, Ahmed Mahfouz, Rosalie G. J. Rietjens, Roel Bijkerk et al. Analyzing cell-type-specific dynamics of metabolism in kidney repair Nature Metabolism 8/25/2022 [PMID: 36008550]
• Kyohei Matsuura, Natsumi Maehara, Aika Hirota, Ayaka Eguchi, Keisuke Yasuda, Kaori Taniguchi et al. Two independent modes of kidney stone suppression achieved by AIM/CD5L and KIM-1 Communications Biology 2022-08-03 [PMID: 35922481] (Immunohistochemistry, Mouse)
• Kyohei Matsuura, Natsumi Maehara, Aika Hirota, Ayaka Eguchi, Keisuke Yasuda, Kaori Taniguchi et al. Two independent modes of kidney stone suppression achieved by AIM/CD5L and KIM-1 Communications Biology 8/3/2022 [PMID: 35922481]
• A Lau, JJ Rahn, M Chappellaz, H Chung, H Benediktss, D Bihan, A von Mässen, A Linkermann, CN Jenne, SM Robbins, DL Senger, IA Lewis, J Chun, DA Muruve Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury Science Advances, 2022-02-02;8(5):eabm0142. 2022-02-02 [PMID: 35108057] (IHC, Mouse)
• T Pongking, O Haonon, R Dangtakot, S Onsurathum, A Jusakul, K Intuyod, A Sangka, S Anutrakulc, U Cha'on, S Pinlaor, P Pinlaor A combination of monosodium glutamate and high-fat and high-fructose diets increases the risk of kidney injury, gut dysbiosis and host-microbial co-metabolism PLoS ONE, 2020-04-08;15(4):e0231237. 2020-04-08 [PMID: 32267892] (IHC, Hamster)
• Y Nozaki, J Ri, K Sakai, K Niki, M Funauchi, I Matsumura Protective Effects of Recombinant Human Soluble Thrombomodulin on Lipopolysaccharide-Induced Acute Kidney Injury Int J Mol Sci, 2020-04-05;21(7):. 2020-04-05 [PMID: 32260474] (IHC, Mouse)
• JA Manning, SS Shah, TL Henshall, A Nikolic, J Finnie, S Kumar Dietary sodium modulates nephropathy in Nedd4-2-deficient mice Cell Death Differ., 2019-12-04;0(0):. 2019-12-04 [PMID: 31802037] (IHC-Fr, Mouse)
• Takuto Chiba, Kevin D. Peasley, Kasey R. Cargill, Katherine V. Maringer, Sivakama S. Bharathi, Elina Mukherjee et al. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI Journal of the American Society of Nephrology 12/1/2019 [PMID: 31575700]
• Ming-Zhi Zhang, Kensuke Sasaki, Yan Li, Zhilian Li, Yu Pan, Guan-nan Jin et al. The Role of the EGF Receptor in Sex Differences in Kidney Injury Journal of the American Society of Nephrology 9/1/2019 [PMID: 31292196]
• Jianchun Chen, Huaizhou You, Yan Li, You Xu, Qian He, Raymond C. Harris EGF Receptor–Dependent YAP Activation Is Important for Renal Recovery from AKI Journal of the American Society of Nephrology 9/1/2018 [PMID: 30072422]
• A Lau, H Chung, T Komada, JM Platnich, CF Sandall, SR Choudhury, J Chun, V Naumenko, BG Surewaard, MC Nelson, A Ulke-Lemée, PL Beck, H Benediktss, AM Jevnikar, SL Snelgrove, MJ Hickey, DL Senger, MT James, JA Macdonald, P Kubes, CN Jenne, DA Muruve Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury J. Clin. Invest., 2018-06-04;0(0):. 2018-06-04 [PMID: 29863495] (IHC-P, Mouse)
• Tanya L Henshall, Jantina A Manning, Omri S Alfassy, Pranay Goel, Natasha A Boase, Hiroshi Kawabe et al. Deletion of Nedd4-2 results in progressive kidney disease in mice Cell Death & Differentiation 12/1/2017 [PMID: 28862701]
• Zhang Ming-Zhi, Wang Xin, Wang Yinqiu et al. IL-4/IL-13-mediated polarization of renal macrophages/dendritic cells to an M2a phenotype is essential for recovery from acute kidney injury. Kidney International 1/1/2017 [PMID: 27745702]
• Wang Y, Chang J, Yao B, Niu A, Kelly E, Breeggemann M, Abboud Werner S, Harris R, Zhang M Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury. Kidney Int, 2015-09-30;88(6):1274-1282. 2015-09-30 [PMID: 26422503] (IHC-P, Mouse)
• Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J KIM-1-mediated phagocytosis reduces acute injury to the kidney. J Clin Invest, 2015-03-09;125(4):1620-36. 2015-03-09 [PMID: 25751064] (ELISA Development (Capture), Mouse)
• Yano T, Nozaki Y, Kinoshita K, Hino S, Hirooka Y, Niki K, Shimazu H, Kishimoto K, Funauch M, Matsumura I The pathological role of IL-18Ralpha in renal ischemia/reperfusion injury. Lab Invest, 2014-10-20;95(1):78-91. 2014-10-20 [PMID: 25329004] (IHC-Fr, Mouse)
• Venkata S. Sabbisetti, Sushrut S. Waikar, Daniel J. Antoine, Adam Smiles, Chang Wang, Abinaya Ravisankar et al. Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes Journal of the American Society of Nephrology 2014-10-01 [PMID: 24904085] (ELISA Capture, Mouse)
• Cuadros T, Trilla E, Sarro E, Vila M, Vilardell J, de Torres I, Salcedo M, Lopez-Hellin J, Sanchez A, Ramon y Cajal S, Itarte E, Morote J, Meseguer A HAVCR/KIM-1 activates the IL-6/STAT-3 pathway in clear cell renal cell carcinoma and determines tumor progression and patient outcome. Cancer Res, 2014-01-03;74(5):1416-28. 2014-01-03 [PMID: 24390735] (ICC, Human)
• Zhang MZ, Yao B, Yang S et al. CSF-1 signaling mediates recovery from acute kidney injury J Clin Invest 12/3/2012 [PMID: 23143303]
• Nozaki Y, Nikolic-Paterson DJ, Snelgrove SL Endogenous Tim-1 (Kim-1) promotes T-cell responses and cell-mediated injury in experimental crescentic glomerulonephritis. Kidney Int., 2011-12-28;81(9):844-55. 2011-12-28 [PMID: 22205357] (ELISA Development, Western Blot, Mouse)
• Ming-Zhi Zhang, Bing Yao, Suwan Wang, Xiaofeng Fan, Guanqing Wu, Haichun Yang et al. Intrarenal dopamine deficiency leads to hypertension and decreased longevity in mice Journal of Clinical Investigation 7/1/2011 [PMID: 21701066]
• Ming-Zhi Zhang, Bing Yao, Suwan Wang, Xiaofeng Fan, Guanqing Wu, Haichun Yang et al. Intrarenal dopamine deficiency leads to hypertension and decreased longevity in mice Journal of Clinical Investigation 2011-07-01 [PMID: 21701066] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Kim HS, Kim HS, Lee CW, Chung DH T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production. J. Immunol., 2010-03-10;184(8):4095-106. 2010-03-10 [PMID: 20220086] (Flow Cytometry, Mouse)
• Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood, 2007-07-09;110(7):2565-8. 2007-07-09 [PMID: 17620455] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • NP_001160104()