TIM-1/KIM-1/HAVCR Antibody (219211) [Phycoerythrin]

(A) Unstimulated and (B) Th2-stimulated human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR PE-conjugated Monoclonal Antibody (Catalog # FAB1750P) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

TIM-1/KIM-1/HAVCR Antibody (219211) [Phycoerythrin] Summary

• Additional Information: Clone 219211 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR
  Ser21-Thr288
  Accession # AAC39862
• Specificity: Detects human TIM-1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAVCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-1/KIM-1/HAVCR Antibody (219211) [Phycoerythrin]

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM-1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
4. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
5. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
6. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
7. Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
8. Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (FAB1750P)(1)

Publication using FAB1750P

• LW Chu, CJ Yang, KJ Peng, PL Chen, SJ Wang, YH Ping TIM-1 As a Signal Receptor Triggers Dengue Virus-Induced Autophagy Int J Mol Sci, 2019-10-02;20(19):. 2019-10-02 [PMID: 31581681] (Confocal Microscopy, ICC, Human)

Bioinformatics

• Gene Symbol: HAVCR1
• Uniprot:
  • AAC39862()