TIGIT Antibody (2629A) [Unconjugated]

Images

 
In a functional ELISA, Recombinant Cynomolgus Monkey TIGIT (9380-TG) binds to immobilized Recombinant Human CD155/PVR (2530-CD) coated at 2.5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is ...read more
HEK293 human embryonic kidney cell line transfected with either (A) cynomolgus monkey TIGIT or (B) irrelevant protein, and eGFP, was stained with Rabbit anti-cynomolgus monkey TIGIT monoclonal antibody (Catalog # ...read more

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
2629A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TIGIT Antibody (2629A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human embryonic kidney cell HEK293-derived Cynomolgus monkey TIGIT
Met22-Pro142
Accession # XP_00554815
Specificity
Detects Cynomolgus Monkey TIGIT in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction 70-350 ng/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIGIT Antibody (2629A) [Unconjugated]

  • T cell immunoreceptor with Ig and ITIM domains
  • TIGIT
  • VSIG9
  • VSTM3
  • WUCAM

Background

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called VSTM3 (V-set and transmembrane domain-containing 3), VSIG9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. Cyno TIGIT shares 88.4% homology with human TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).
  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. SwissProt Accession # Q495A1.
  6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB10532U
Alexa Fluor 405 FAB10532V
Alexa Fluor 488 FAB10532G
Alexa Fluor 532 FAB10532X
Alexa Fluor 594 FAB10532T
Alexa Fluor 647 FAB10532R
Alexa Fluor 700 FAB10532N
Alexa Fluor 750 FAB10532S
Allophycocyanin FAB10532A
Biotin FAB10532B
DyLight 350 FAB10532D
DyLight 405 FAB10532E
DyLight 488 FAB10532K
DyLight 550 FAB10532L
DyLight 594 FAB10532M
DyLight 650 FAB10532W
DyLight 680 FAB10532Y
DyLight 755 FAB10532Z
FITC FAB10532F
Janelia Fluor 549 FAB10532I
Janelia Fluor 646 FAB10532J
PE FAB10532P
PerCP FAB10532C

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