Mouse Tight Junction Protein 1 ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Mouse Tight Junction Protein 1, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse Tight Junction Protein 1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Specificity: This kit recognizes Mouse Tight Junction Protein 1 in samples. No significant cross-reactivity or interference between Mouse Tight Junction Protein 1 and analogues was observed
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TJP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Tight Junction Protein 1 ELISA Kit (Colorimetric)

• DKFZp686M05161
• MGC133289
• tight junction protein 1 (zona occludens 1)
• Tight junction protein 1
• tight junction protein ZO-1
• TJP1
• ZO1
• ZO-1
• zona occludens 1
• Zona occludens protein 1
• zonula occludens 1 protein
• Zonula occludens protein 1

Background

Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Tight Junction Protein 1 ELISA Kit (NBP3-49965). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: TJP1