Tight Junction Protein 1 Antibody

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu
• Applications: ELISA, ICC/IF, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tight Junction Protein 1 Antibody Summary

• Description: Novus Biologicals Goat Tight Junction Protein 1 Antibody (NBP1-46111) is a polyclonal antibody validated for use in ELISA and ICC/IF. Anti-Tight Junction Protein 1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PKPTSQNQFSEHDKT corresponding to internal region according to NP_003248.3, NP_783297.2.
• Epitope: internal region
• Marker: Intercellular Junctions/Tight Junction Marker
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TJP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:132000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tight Junction Protein 1 Antibody

• DKFZp686M05161
• MGC133289
• tight junction protein 1 (zona occludens 1)
• Tight junction protein 1
• tight junction protein ZO-1
• TJP1
• ZO1
• ZO-1
• zona occludens 1
• Zona occludens protein 1
• zonula occludens 1 protein
• Zonula occludens protein 1

Background

Zona Occludens or tight junctions are plasma membrane structures that seal together the perimeters of polarized epithelial cell monolayers and provide the cellular barrier necessary to maintain vectoral secretion, absorption and transport; often defining luminal and intracellular space. Four tight junction specific proteins have been identified (ZO1, ZO2, ZO3 and occludin). ZO1 was the first protein shown to be specifically associated with the tight junction and remains the standard marker for these structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tight Junction Protein 1 Antibody (NBP1-46111)(2)

Publications using NBP1-46111

• Cheng Y, Medina A, Yao Z et al. Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model Cell reports 2022-05-31 [PMID: 35649379] (ICC/IF, Human)
• Zhong Z, Deane R, Ali Z et al. ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat Neurosci 2008-04-01 [PMID: 18344992]

Bioinformatics

• Gene Symbol: TJP1
• Entrez:
  • 7082(Human)
• Uniprot:
  • NP_003248.3(Human)