TIGAR/C12orf5 Antibody

Product Discontinued

TIGAR/C12orf5 Antibody Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the center of human TIGAR. The immunogen is located within amino acids 100 - 150 of TIGAR.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIGAR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIGAR/C12orf5 Antibody

• C12orf5
• chromosome 12 open reading frame 5
• EC 3.1.3.46
• probable fructose-2,6-bisphosphatase TIGAR
• TIGAR
• TIGARTP53-induced glycolysis and apoptosis regulator
• transactivated by NS3TP2 protein

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIGAR
• Entrez:
  • 57103(Human)
• Uniprot:
  • Q9NQ88(Human)