TIF1 gamma Antibody

Western Blot: TIF1 gamma Antibody [NB100-57498] - Detection of Mouse TRIM33/ TIF1gamma by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-TRIM33/ TIF1gamma antibody NB100-57498 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NB100-57498] - Section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- TRIM33/TIF1gamma used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: TIF1 gamma Antibody [NB100-57498] - Detection of Human TIF1gamma on HeLa whole cell lysate using NB100-57498. TIF1gamma was also immunoprecipitated by rabbit anti-TIF1gamma antibody NB100-57497.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TIF1 gamma Antibody Summary

• Description: Novus Biologicals Rabbit TIF1 gamma Antibody (NB100-57498) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TIF1 gamma Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1077 and the C-terminus (residue 1127) of human tripartite motif-containing 33 (transcriptional intermediary factor 1 gamma) using the numbering given in entry NP_056990.3 (GeneID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM33
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIF1 gamma Antibody

• E3 ubiquitin-protein ligase TRIM33
• EC 6.3.2
• EC 6.3.2.-
• Ectodermin homolog
• KIAA1113
• Protein Rfg7
• PTC7FLJ32925
• RET-fused gene 7 protein
• RFG7FLJ11429
• TF1G
• TIF1GAMMA
• TIF1-gamma
• TIF1GECTO
• TIFGAMMA
• Transcription intermediary factor 1-gamma
• transcriptional intermediary factor 1 gamma
• tripartite motif containing 33
• tripartite motif-containing 33
• Tripartite motif-containing protein 33

Background

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIF1 gamma Antibody (NB100-57498)(1)

Publication using NB100-57498

• Okiyama N, Ichimura Y, Shobo M et al. Immune response to dermatomyositis-specific autoantigen, transcriptional intermediary factor 1 gamma can result in experimental myositis Annals of the rheumatic diseases 2021-04-02 [PMID: 33811031] (WB, Mouse)

Bioinformatics

• Gene Symbol: TRIM33
• Entrez:
  • 51592(Human)
• Uniprot:
  • Q9UPN9(Human)