TIA1 Antibody (TIA1/1352R) [Janelia Fluor® 635]

Product Discontinued

TIA1 Antibody (TIA1/1352R) [Janelia Fluor® 635] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human bone marrow malignant cells from a non B, non T acute leukemia
• Localization: Cytoplasmic granule, Nucleus, Accumulates in cytoplasmic stress granules (SG) following cellular damage
• Specificity: The T cell intracellular antigen 1 (TIA 1) is a 17 kDa cytoplasmic granule associated protein also designated as GMP 17, for granule membrane protein of 17 kDa. The GMP 17/TIA 1 molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95) mediated apoptosis. Within hematopoietic cell lines, the TIA1/1352R monoclonal antibody reacts with about 90% of CD16+, 50 60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T cell clones, activated NK cell clones, and Con activated thymocytes, but not with B lymphocytes or B cell lines. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TIA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TIA1 Antibody (TIA1/1352R) [Janelia Fluor® 635]

• cytotoxic granule-associated RNA-binding protein
• nucleolysin TIA-1 isoform p40
• p40-TIA-1 (containing p15-TIA-1)
• p40-TIA-1
• RNA-binding protein TIA-1
• T-cell-restricted intracellular antigen-1
• TIA1 cytotoxic granule-associated RNA binding protein
• TIA1 cytotoxic granule-associated RNA-binding protein
• TIA-1

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIA1