HuR/ELAVL1 Antibody (4G8)

Western Blot: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - ELAVL1 monoclonal antibody (M02), clone 4G8 Analysis of ELAVL1 expression in HeLa.

Immunocytochemistry/Immunofluorescence: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Analysis of monoclonal antibody to ELAVL1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Analysis of monoclonal antibody to ELAVL1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.

Sandwich ELISA: HuR/ELAVL1 Antibody (4G8) [H00001994-M02] - Detection limit for recombinant GST tagged ELAVL1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 4G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HuR/ELAVL1 Antibody (4G8) Summary

• Immunogen: ELAVL1 (AAH03376, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS
• Specificity: ELAVL1 - ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELAVL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA, IF and IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HuR/ELAVL1 Antibody (4G8)

• ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• ELAV-like protein 1
• embryonic lethal, abnormal vision, drosophila, homolog-like 1
• Hu antigen R
• Hua
• Hu-antigen R
• HuR
• HURELAV1
• MelG

Background

The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HuR/ELAVL1 Antibody (H00001994-M02)(1)

Publication using H00001994-M02

• Kani K, Faca VM, Hughes LD et al. Quantitative Proteomic profiling identifies protein correlates to EGFR kinase inhibition. Mol Cancer Ther. 2012-03-21 [PMID: 22411897]

Review for HuR/ELAVL1 Antibody (H00001994-M02) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/14/2017

Summary

• Application: Western Blot
• Sample Tested: 3 human cancer cell lines
• Species: Human
• Lot: G6161-4G8

Comments

• Comments: three tumor cell line lysates (10 ug) are loeded.
  1/2000 dil in 2% BSA/TBST of Ab, incubate for o/n at 4C

Bioinformatics

• Gene Symbol: ELAVL1
• Entrez:
  • 1994(Human)
• OMIM:
  • 603466(Human)
• Uniprot:
  • AAH03376(Human)