Thymidylate Synthase Antibody (TYMS/9091R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/9091R) - Azide and BSA Free [NBP3-24023] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Thymidylate Synthase Antibody (TYMS/9091R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/9091R) - Azide and BSA Free [NBP3-24023] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Thymidylate Synthase Antibody (TYMS/9091R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: TYMS/9091R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Thymidylate Synthase Antibody (TYMS/9091R) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human Thymidylate Synthase protein fragment (around aa 1-200) (exact sequence is proprietary)
• Localization: Nucleus. Cytoplasm.
• Marker: 5-FU Resistance Marker
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TYMS
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Thymidylate Synthase Antibody (TYMS/9091R) - Azide and BSA Free

• EC 2.1.1.45
• HsT422
• MGC88736
• OK/SW-cl.29
• Thymidylate Synthase
• thymidylate synthetase
• TMSTsase
• TS
• TSase
• TSHST422
• TYMS

Background

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYMS