Thrombospondin-1 Antibody [Unconjugated]

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Western blot shows lysates of MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) ...read more
Thrombospondin‑1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Thrombospondin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3074) at 1.7 µg/mL ...read more
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin‑1 at approximately 221 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Thrombospondin-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-1
Asn19-Pro1170
Accession # CAA32889
Specificity
Detects human Thrombospondin-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THBS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3074 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombospondin-1 Antibody [Unconjugated]

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • Thrombospondin1
  • Thrombospondin-1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1

Background

Thrombospondin-1 (TSP-1) is a 150‑180 kDa charter member of the thrombospondin family of extracellular matrix proteins. Human TSP‑1 is synthesized as an 1170 amino acid (aa) precursor that contains an 18 aa signal sequence and 1152 aa mature molecule. The mature molecule has been described as containing three distinct regions that create the shape of a dumbbell. There is an intital, 140 aa N-terminal laminin G-like globular region that binds heparin (aa 19‑258). This is followed by an extended, central collagen-binding region that contains one type C von Willebrand factor domain, plus three TSP type I and three TSP type II (or EGF-like) domains (aa 259‑712). The C‑terminus (aa 713‑1170) appears as a large globule with two halves; one calcium‑binding region (aa 713‑950) with seven Asp‑rich TSP type III domains, and one terminal region (aa 951‑1170) with TSP‑unique motifs (1). This C-terminal region is believed to mediate CD47 and cell binding (2‑5). The TSP type I repeats have multiple functions. They bind to type V collagen, laminin, fibronectin and CD36. They also contain a recognition site for C‑mannosylation on Trp. Finally, a type I KRFK motif induces the release of mature TGF-b from LAP. This is an effect not found in TSP-2. The function of the type II repeats in unclear. TSP‑1 is secreted as a disulfide-linked 450 kDa homotrimer. The cysteines responsible lie just N‑terminal to the first type I TSP repeat. Mature human TSP-1 is 61% aa identical to human TSP-2. It is also 95%, 97% and 95% aa identical to mouse, dog and rat TSP-1, respectively.

  1. Lawler, J. and R.O. Hynes (1986) J. Cell Biol. 103:1635.
  2. Frazier, W.A. (1987) J. Cell Biol. 105:625.
  3. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  4. Sid, B. et al. (2004) Crit. Rev. Oncol. Hematol. 49:245.
  5. Floquet, N. et al. (2008) Anch. Biochem. Biophys. 478:103.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thrombospondin-1 Antibody (AF3074)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(4)
Rat
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF3074 Applications Species
A Bhattachar, MN Janal, R Veeramacha, I Dolgalev, Z Dubeykovsk, NH Tu, H Kim, S Zhang, AK Wu, M Hagiwara, AR Kerr, MD DeLacure, BL Schmidt, DG Albertson Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes Sci Rep, 2020;10(1):14724. 2020 [PMID: 32895418] (Western Blot, Human) Western Blot Human
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019;28(13):3329-3337.e5. 2019 [PMID: 31553904] (Western Blot, Human) Western Blot Human
X Miao, MF Rahman, L Jiang, Y Min, S Tan, H Xie, L Lee, M Wang, RE Malmström, WO Lui, N Li Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1 J. Thromb. Haemost., 2018;0(0):. 2018 [PMID: 29442415] (Western Blot, Human) Western Blot Human
S Koh, WJ Chen, NS Dejneka, IR Harris, B Lu, S Girman, J Saylor, S Wang, C Eroglu Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates M�ller Glial Reactivity J. Neurosci., 2018;0(0):. 2018 [PMID: 29431645] (IHC-P, Rat) IHC-P Rat
Villarreal G, Chatterjee A, Oh S, Oh D, Kang M, Rhee D Canonical wnt signaling regulates extracellular matrix expression in the trabecular meshwork. Invest Ophthalmol Vis Sci, 2015;55(11):7433-40. 2015 [PMID: 25352117] (Western Blot, Human) Western Blot Human

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Biotin BAF3074

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Bioinformatics

Gene Symbol THBS1
Uniprot