Recombinant Human Thrombopoietin/THPO Protein

Product Discontinued

Recombinant Human Thrombopoietin/THPO Protein Summary

• Description: Recombinant bioactive protein for Human Thrombopoietin/Tpo
  
  Source:E. coli
  Amino Acid Sequence:(P07202)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab285559)
• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/ml, corresponding to a specific activity of >1 x 106 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: THPO
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be <1 ng/ml, corresponding to a specific activity of >1 x 10^6 IU/mg.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized with no additives
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in purified H2O to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage. For long term storage add a carrier protein (0.1% HSA or BSA).

Alternate Names for Recombinant Human Thrombopoietin/THPO Protein

• Megakaryocyte colony-stimulating factor
• Megakaryocyte growth and development factor
• megakaryocyte stimulating factor
• MGDF
• MGDFC-mpl ligand
• MKCSF
• MK-CSF
• ML
• MPL ligand
• MPLLG
• MPLLGMGC163194
• Myeloproliferative leukemia virus oncogene ligand
• THCYT1
• THPO
• thrombopoietin nirs variant 1
• Thrombopoietin
• Tpo
• TPOMKCSF

Background

TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide, which contains the erythropoietin-like domain of the full length TPO protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: THPO