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488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND50 is typically 0.1-0.3 µg/mL." class="big_lightbox" target="_blank">
488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA). The ND50 is typically 0.05-0.3 µg/mL." class="big_lightbox" target="_blank">
288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.