488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox"> 488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." alt="Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_lightbox"> 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." alt="Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_thumb" />

288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND₅₀ is typically 5-10 µg/mL." class="big_lightbox"> 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND₅₀ is typically 5-10 µg/mL." alt="Recombinant Human Thrombo-poietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human mega-karyocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombo-poietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombo-poietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND₅₀ is typically 5-10 µg/mL." class="big_thumb" />

288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 288-TPN) was serially diluted and captured by Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-288-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) was serially diluted and captured by Rat Anti-Mouse Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB488) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Thrombopoietin/Tpo was detected in immersion fixed paraffin-embedded sections of breast cancer using Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB2882) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for Thrombopoietin/Tpo at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Thrombopoietin/Tpo at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB11609) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thrombopoietin/Tpo at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />