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Thrombomodulin/BDCA-3 Antibody (QBEND/40)

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Immunocytochemistry/Immunofluorescence: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Analysis of human tonsil cryosection.
Immunocytochemistry/ Immunofluorescence: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Analysis of human tonsil cryosection.
Immunohistochemistry-Frozen: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Analysis of human spleen.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Human lymph node.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
QBEND/40
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Thrombomodulin/BDCA-3 Antibody (QBEND/40) Summary

Immunogen
Human endothelium (thrombomodulin)
Specificity
Recognizes all human endothelium tested. Preliminary evidence suggests that this recognizes Thrombomodulin, but does not inhibit Thrombomodulin dependent activation of protein C.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
THBD
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Flow Cytometry Neat
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunohistochemistry-Paraffin 1:10-1:100
Application Notes
This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate pH6.0 is recommended for this purpose.

Reactivity Notes

Reacts with: Rabbit

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2M Tris/HCl (pH 7.4) and 5 - 10% fetal Bovine serum (FBS)
Preservative
0.01% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Thrombomodulin/BDCA-3 Antibody (QBEND/40)

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM

Background

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol THBD
Entrez
Uniprot