Thrombomodulin/BDCA-3 Antibody (QBEND/40)

Immunocytochemistry/Immunofluorescence: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Analysis of human tonsil cryosection.

Immunohistochemistry-Frozen: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Analysis of human spleen.

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (QBEND/40) [NB100-64982] - Human lymph node.

Product Discontinued

Thrombomodulin/BDCA-3 Antibody (QBEND/40) Summary

• Immunogen: Human endothelium (thrombomodulin)
• Specificity: Recognizes all human endothelium tested. Preliminary evidence suggests that this recognizes Thrombomodulin, but does not inhibit Thrombomodulin dependent activation of protein C.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: THBD
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Flow Cytometry Neat
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
  • Immunohistochemistry-Paraffin 1:10-1:100
• Application Notes: This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Sodium Citrate pH6.0 is recommended for this purpose.

Reactivity Notes

Reacts with: Rabbit

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 M Tris-HCl (pH 7.4), 5 - 10% Fetal Bovine Serum (FBS)
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Thrombomodulin/BDCA-3 Antibody (QBEND/40)

• BDCA3
• BDCA-3
• CD141 antigen
• CD141
• Fetomodulin
• THBD
• THRM
• Thrombomodulin
• TM

Background

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THBD
• Entrez:
  • 7056(Human)
• Uniprot:
  • P07204(Mouse)