Thioredoxin Reductase 1/TRXR1 Antibody

Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - 50 ug Rat brain lysate/extract 7. 5% SDS-PAGE TrxR1 antibody [N3C2], Internal dilution: 1:1000.

Immunocytochemistry/Immunofluorescence: thioredoxin reductase 1 Antibody [NBP2-20619] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20619] - TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse liver by Immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TrxR1 antibody [N3C2], Internal diluted at 1:500.

Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - Sample (30 ug of whole cell lysate)A: Molt-4 7. 5% SDS PAGE gel, diluted at 1:3000.

Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - 50 ug mouse brain lysate/extract 7. 5% SDS-PAGE TrxR1 antibody [N3C2], Internal dilution: 1:1000.

Immunohistochemistry-Paraffin: thioredoxin reductase 1 Antibody [NBP2-20619] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Thioredoxin Reductase 1/TRXR1 Antibody [NBP2-20619] - TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. TrxR1 antibody [N3C2], Internal diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Pm, Po, Bv, Ca, Pm, Rb, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Thioredoxin Reductase 1/TRXR1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.
• Localization: Cytoplasm; Isoform 4: Cytoplasm; Nucleus; Isoform 5: Cytoplasm
• Predicted Species: Porcine (93%), Bovine (93%), Rabbit (96%), Rhesus Macaque (98%), Canine (96%), Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TXNRD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Monkey reactivity reported in scientific literature (Liang Z et al).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Thioredoxin Reductase 1/TRXR1 Antibody

• EC 1.8.1
• EC 1.8.1.9
• Gene associated with retinoic and IFN-induced mortality 12 protein
• Gene associated with retinoic and interferon-induced mortality 12 protein
• GRIM12
• GRIM-12
• GRIM-12TR1
• KDRF
• KM-102-derived reductase-like factor
• MGC9145
• oxidoreductase
• Thioredoxin Reductase 1
• thioredoxin reductase 1, cytoplasmic
• thioredoxin reductase GRIM-12
• Thioredoxin reductase TR1
• TR
• TR1
• TRXR1
• TRXRD1
• TXNR
• TXNRD1
• TXNRTRXR1

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Thioredoxin Reductase 1/TRXR1 Antibody (NBP2-20619)(1)

Publication using NBP2-20619

• Liang Z, Zhu B, Meng D et al. Evaluation of the Effect of Carvedilol on the Thioredoxin Pathway in H9C2 Rat Cardiomyocytes Thesis (WB, IP, Monkey)
  
  Details:
  Green monkey

Bioinformatics

• Gene Symbol: TXNRD1
• Uniprot:
  • Q16881()