Themis Antibody (701010)

Human peripheral blood lymphocytes were stained with Mouse Anti-Human Themis Monoclonal Antibody (Catalog # MAB6929) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 701010
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Themis Antibody (701010) Summary

• Immunogen: E. coli-derived recombinant human Themis
  Ala2-Pro282
  Accession # Q8N1K5
• Specificity: Detects human Themis in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Themis is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: THEMIS
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Themis Antibody (701010)

• bA325O24.3
• bA325O24.4
• C6orf190
• C6orf207
• FLJ40584
• Gasp
• GRB2-associated protein
• MGC163388
• protein THEMIS
• signaling phosphoprotein specific for T cells
• SPOT
• Themis
• thymocyte selection associated
• thymocyte selection pathway associated
• thymocyte-expressed molecule involved in selection
• TSEPA

Background

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, human THEMIS shares 86% aa sequence identity with mouse and rat THEMIS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THEMIS
• Uniprot:
  • Q8N1K5(Human)