THAP11 Antibody (3F3)

Western Blot: THAP11 Antibody (3F3) [NBP1-51638] - Analysis using THAP11 mouse mAb against Hela (1) and NTERA-2 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: THAP11 Antibody (3F3) [NBP1-51638] - Analysis of NTERA-2 cells using THAP11 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: THAP11 Antibody (3F3) [NBP1-51638] - Analysis of paraffin-embedded colon cancer tissues (left) and ovary cancer tissues (right) using THAP11 mouse mAb with DAB staining.

Flow Cytometry: THAP11 Antibody (3F3) [NBP1-51638] - Analysis of Hela cells using THAP11 mouse mAb (green) and negative control (purple).

Product Discontinued

THAP11 Antibody (3F3) Summary

• Immunogen: Purified recombinant fragment of human THAP11 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: THAP11
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for THAP11 Antibody (3F3)

• CTG-B43a
• CTG-B45d
• HRIHFB2206
• Ronin
• THAP domain containing 11
• THAP domain-containing protein 11
• THAP11

Background

THAP11 is a member of the thanatos-associated proteins (THAP) family, which are zinc-dependent, sequence-specific DNA-binding factors involved in cell proliferation, apoptosis, cell cycle, chromatin modification and transcriptional regulation (Zhu, et al). THAP11 has been hypothesized to be a cell growth inhibitor via the repression of c-myc (Zhu, et al), and THAP11 expression has also been linked to the proliferation of embryonic stem cells (Dejosez M et al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THAP11