TGN46 Antibody

Images

 
Western Blot: TGN46 Antibody [NBP1-86949] - Analysis in control (vector only transfected HEK293T lysate) and TGOLN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: TGN46 Antibody [NBP1-86949] - Staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Western Blot: TGN46 Antibody [NBP1-86949] - Analysis of lysate from 293T cells. Image from verified customer review.
Independent Antibodies: Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex, colon, kidney and testis using Anti-TGOLN2 antibody NBP1-86949 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human kidney.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human testis.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human colon.
Immunohistochemistry-Paraffin: TGN46 Antibody [NBP1-86949] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

TGN46 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ATESVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSSAEAQTPEDTPNKSGAEAKTQKDSSNRSGA
Marker
TGN Marker
Specificity
Specificity of human TGN46 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGOLN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation
Application Notes
IP, WB, ICC/IF reported in scientific literature (PMID: 23532850). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Reviewed Applications
Read 1 Review rated 5
using
NBP1-86949 in the following applications:

Publications
Read Publication using
NBP1-86949 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23532850).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TGN46 Antibody

  • TGN 46
  • TGN 48
  • TGN 51
  • TGN38 homolog
  • TGN38
  • TGN-38
  • TGN46
  • TGN-46
  • TGN48
  • TGN51
  • TGOLN 2
  • TGOLN2
  • trans golgi network 38
  • trans golgi network 46
  • Trans Golgi network integral membrane protein 2 [Precursor]
  • Trans Golgi network integral membrane protein 2
  • Trans Golgi network protein (46 48 51kD isoforms)
  • Trans golgi network protein 2
  • Trans Golgi network protein TGN51
  • Trans-Golgi network integral membrane protein 2
  • Trans-Golgi network protein 2
  • Trans-Golgi network protein TGN51
  • TTGN 2
  • TTGN2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGN46 Antibody (NBP1-86949)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(1)
IP
(1)
WB
(1)
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Review for TGN46 Antibody (NBP1-86949) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-86949:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TGN46 NBP1-86949
Enlarge
reviewed by:
Anonymous
WB Human 09/05/2014
View

Summary

ApplicationWestern Blot
Sample TestedLysate from 293T cells
SpeciesHuman

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 10 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN46 Antibody (NBP1-86949) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional TGN46 Products

Array NBP1-86949

Bioinformatics Tool for TGN46 Antibody (NBP1-86949)

Discover related pathways, diseases and genes to TGN46 Antibody (NBP1-86949). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TGN46 Antibody (NBP1-86949)

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PTMs for TGN46 Antibody (NBP1-86949)

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Research Areas for TGN46 Antibody (NBP1-86949)

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Blogs on TGN46

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Recent Reviews

5
5
1
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0
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0
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1
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Anonymous
09/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TGOLN2