TGN38 Antibody

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TGN38 Antibody Summary

• Description: Novus Biologicals Sheep TGN38 Antibody (NBP1-20263) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TGN38 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen.
• Marker: TGN Marker
• Specificity: TGN38 (or TGN46, TGN48, TGN51 in human).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TGOLN2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000
  • Western Blot 1:1000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID: 25278553).

Reactivity Notes

Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TGN38 Antibody

• TGN38
• TGN46
• TGN46TGN38 homolog
• TGN48MGC14722
• TGN51
• TGN5148, 51kD isoforms)
• Tgoln1
• Tgoln2
• trans-golgi network protein 2
• Trans-Golgi network protein TGN51
• Ttgn1

Background

FUNCTION: May be involved in regulating membrane traffic to and from trans-Golgi network.; SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Note: Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.; TISSUE SPECIFICITY: Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.; MISCELLANEOUS: Not found in strains BALB/c, C57BL/6 and DBA/2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN38 Antibody (NBP1-20263)(4)

Publications using NBP1-20263

• Andreeva L, David L, Rawson S et al. NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation Cell 2021-12-22 [PMID: 34861190]
• Cornejo VH, GonzAlez C, Campos M et al. Non-conventional Axonal Organelles Control TRPM8 Ion Channel Trafficking and Peripheral Cold Sensing Cell Rep 2020-03-31 [PMID: 32234483] (ICC/IF, Mouse)
• DiCello J J, Saito A et al. Inflammation-associated changes in DOR expression and function in the mouse colon. Am J Physiol Gastrointest Liver Physiol 2018-01-10 [PMID: 29927325] (IF/IHC, Mouse)
• Anitei M, Chenna R, Czupalla C et al. A high throughput siRNA screen identifies genes that regulate mannose 6-phosphate receptor trafficking. J. Cell. Sci. 2014-10-02 [PMID: 25278553] (ICC/IF, Human)
  
  Details:
  TGN38 antibody used for ICC-IF on HeLa cells stably expressing a chimeric protein comprising the GFP fused to the transmembrane and cytoplasmic domain of the cation-independent mannose 6-phosphate receptor /GFP?MPR.

Review for TGN38 Antibody (NBP1-20263) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 12/23/2014

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23913690
• Species: Mouse

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
  • 22135(Mouse)
  • 192152(Rat)
• Uniprot:
  • Q62314(Mouse)