TGN38 Antibody

Western blot shows lysates of RBL-2H3 rat basophilic leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TGN38 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TGN38 was detected in immersion fixed RBL-2H3 rat basophilic leukemia cell line using Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi vesicles in cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TGN38 Antibody Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant rat TGN38
  Leu18-Ser303
  Accession # P19814
• Specificity: Detects rat TGN38 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse TGN38 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Tgoln2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGN38 Antibody

• TGN38
• TGN46
• TGN46TGN38 homolog
• TGN48MGC14722
• TGN51
• TGN5148, 51kD isoforms)
• Tgoln1
• Tgoln2
• trans-golgi network protein 2
• Trans-Golgi network protein TGN51
• Ttgn1

Background

TGN38 (Trans-Golgi network integral membrane protein TGN38) is an 80-100 kDa integral membrane protein that is associated with intracellular trafficking. Its name derives from the fact that its predicted MW is 38 kDa. Although TGN38 cycles continuously between the Golgi and plasma membrane via endosomal vesicles, it is principally localized to the Golgi apparatus. Functionally, TGN38 is involved in both the formation of secretory vesicles, and in the process of clathrin-mediated endocytosis. This is accomplished through the creation of a 250 kDa cytoplasmic complex composed of a Tgoln1 dimer, Rab6, and other molecules that interact with the F-actin binding proteins neurabin-I and -II. This interaction likely contributes to directional vesicle trafficking. Mature rat Tgoln1 is a type I transmembrane glycoprotein that is 340 amino acids (aa) in length. It contains a 286 aa extracellular/luminal region (aa 18-303) plus a 33 aa cytoplasmic domain (aa 325-357). The luminal region appears to be heavily glycosylated with both O- and N-linked carbohydrate, some of which terminates in polysialylation; the cytoplasmic domain contains a cytosolic trafficking motif that encompases aa 348-353. There is one splice variant (TGN41) that contains a 26 aa substitution for the three C-terminal aa of TGN38. It has been suggested that this may heterodimerize with TGN38. Over aa 18-303, rat TGN38/Tgoln1 shares 67% aa sequence identity with the mouse ortholog to rat Tgoln1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Tgoln2
• Uniprot:
  • P19814()