TGN38 Antibody

Images

 
Western blot shows lysates of RBL-2H3 rat basophilic leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) followed by ...read more
TGN38 was detected in immersion fixed RBL-2H3 rat basophilic leukemia cell line using Sheep Anti-Rat TGN38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8059) at 1.7 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

TGN38 Antibody Summary

Immunogen
Human embryonic kidney cell line HEK293-derived recombinant rat TGN38
Leu18-Ser303
Accession # P19814
Specificity
Detects rat TGN38 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse TGN38 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Tgoln2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGN38 Antibody

  • TGN38
  • TGN46
  • TGN46TGN38 homolog
  • TGN48MGC14722
  • TGN51
  • TGN5148, 51kD isoforms)
  • Tgoln1
  • Tgoln2
  • trans-golgi network protein 2
  • Trans-Golgi network protein TGN51
  • Ttgn1

Background

TGN38 (Trans-Golgi network integral membrane protein TGN38) is an 80-100 kDa integral membrane protein that is associated with intracellular trafficking. Its name derives from the fact that its predicted MW is 38 kDa. Although TGN38 cycles continuously between the Golgi and plasma membrane via endosomal vesicles, it is principally localized to the Golgi apparatus. Functionally, TGN38 is involved in both the formation of secretory vesicles, and in the process of clathrin-mediated endocytosis. This is accomplished through the creation of a 250 kDa cytoplasmic complex composed of a Tgoln1 dimer, Rab6, and other molecules that interact with the F-actin binding proteins neurabin-I and -II. This interaction likely contributes to directional vesicle trafficking. Mature rat Tgoln1 is a type I transmembrane glycoprotein that is 340 amino acids (aa) in length. It contains a 286 aa extracellular/luminal region (aa 18-303) plus a 33 aa cytoplasmic domain (aa 325-357). The luminal region appears to be heavily glycosylated with both O- and N-linked carbohydrate, some of which terminates in polysialylation; the cytoplasmic domain contains a cytosolic trafficking motif that encompases aa 348-353. There is one splice variant (TGN41) that contains a 26 aa substitution for the three C-terminal aa of TGN38. It has been suggested that this may heterodimerize with TGN38. Over aa 18-303, rat TGN38/Tgoln1 shares 67% aa sequence identity with the mouse ortholog to rat Tgoln1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol Tgoln2
Uniprot