TGF-beta RII Antibody [Allophycocyanin]

Mouse splenocytes were stained with Goat Anti-Mouse TGF-beta RII APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB532A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

TGF-beta RII Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TGF-beta RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta RII
  Ile24-Asp184
  Accession # Q62312
• Specificity: Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) TGF-beta RII is observed and less than 1% cross-reactivity with recombinant mouse TGF-beta RI and rhTGF-beta RIII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGFBR2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RII Antibody [Allophycocyanin]

• AAT3
• EC 2.7.11
• EC 2.7.11.30
• FAA3
• HNPCC6
• LDS1B
• LDS2B
• MFS2
• RIIC
• TAAD2
• tbetaR-II
• TGF-beta receptor type II
• TGF-beta receptor type IIB
• TGF-beta receptor type-2
• TGF-beta RII
• TGF-beta type II receptor
• TGFbetaRII
• TGFbeta-RII
• TGFBR2
• TGF-bRII
• TGFR-2
• transforming growth factor beta receptor type IIC
• transforming growth factor, beta receptor II (70/80kDa) isoform 1
• transforming growth factor, beta receptor II (70/80kDa) isoform 2
• transforming growth factor, beta receptor II (70/80kDa)
• transforming growth factor, beta receptor II (70-80kD)
• Transforming growth factor-beta receptor type II

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .

1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta RII Antibody (FAB532A)(8)

Publications using FAB532A

• P Chakrabort, RY Parikh, S Choi, D Tran, M Gooz, ZT Hedley, DS Kim, D Pytel, I Kang, SN Nadig, GC Beeson, L Ball, M Mehrotra, H Wang, S Berto, V Palanisamy, H Li, S Chatterjee, PC Rodriguez, EN Maldonado, JA Diehl, VK Gangaraju, S Mehrotra Carbon monoxide activates PERK-regulated autophagy to induce immunometabolic reprogramming and boost anti-tumor T cell function Cancer Research, 2022-05-16;0(0):. 2022-05-16 [PMID: 35404405] (Flow Cytometry, Mouse)
• M Hu, Y Lu, S Wang, Z Zhang, Y Qi, N Chen, M Shen, F Chen, M Chen, L Yang, S Chen, D Zeng, F Wang, Y Su, Y Xu, J Wang CD63 acts as a functional marker in maintaining hematopoietic stem cell quiescence through supporting TGFbeta signaling in mice Cell Death and Differentiation, 2021-08-06;0(0):. 2021-08-06 [PMID: 34363017] (Flow Cytometry, Mouse)
• Henrique Borges Borges da Silva, Changwei Peng, Haiguang Wang, Kelsey M. Wanhainen, Chaoyu Ma, Sharon Lopez et al. Sensing of ATP via the Purinergic Receptor P2RX7 Promotes CD8+ Trm Cell Generation by Enhancing Their Sensitivity to the Cytokine TGF-beta Immunity 7/7/2020 [PMID: 32640257]
• EA Ansa-Addo, Y Zhang, Y Yang, GS Hussey, BV Howley, M Salem, B Riesenberg, S Sun, DC Rockey, S Karvar, PH Howe, B Liu, Z Li Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-? signaling J. Clin. Invest, 2017-03-13;0(0):. 2017-03-13 [PMID: 28287407] (Flow Cytometry, Mouse)
• Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun, 2015-02-19;6(0):6329. 2015-02-19 [PMID: 25695838] (Flow Cytometry, Mouse)
• Zhou S, Dong X, Zhang C, Chen X, Zhu J, Li W, Song X, Xu Z, Zhang W, Yang X, Li Y, Liu F, Sun C MicroRNAs are implicated in the suppression of CD4+CD25- conventional T cell proliferation by CD4+CD25+ regulatory T cells. Mol Immunol, 2015-02-01;63(2):464-72. 2015-02-01 [PMID: 25457879] (Flow Cytometry, Mouse)
• Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis. J Clin Invest, 2013-12-20;124(1):448-60. 2013-12-20 [PMID: 24355923] (Flow Cytometry, Mouse)
• Zhang P, Nakatsukasa H, Tu E, Kasagi S, Cui K, Ishikawa M, Konkel J, Maruyama T, Wei G, Abbatiello B, Wang Z, Zhao K, Chen W PARP-1 regulates expression of TGF-beta receptors in T cells. Blood, 2013-08-12;122(13):2224-32. 2013-08-12 [PMID: 23940283] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TGFBR2
• Uniprot:
  • Q62312()