TGF-beta RII Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

TGF-beta RII Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TGF‑β RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑β RII
  Ile24-Asp184
  Accession # Q62312
• Specificity: Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) TGF-beta RII is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RII Antibody [Alexa Fluor® 405]

• AAT3
• EC 2.7.11
• EC 2.7.11.30
• FAA3
• HNPCC6
• LDS1B
• LDS2B
• MFS2
• RIIC
• TAAD2
• tbetaR-II
• TGF-beta receptor type II
• TGF-beta receptor type IIB
• TGF-beta receptor type-2
• TGF-beta RII
• TGF-beta type II receptor
• TGFbetaRII
• TGFbeta-RII
• TGFBR2
• TGF-bRII
• TGFR-2
• transforming growth factor beta receptor type IIC
• transforming growth factor, beta receptor II (70/80kDa) isoform 1
• transforming growth factor, beta receptor II (70/80kDa) isoform 2
• transforming growth factor, beta receptor II (70/80kDa)
• transforming growth factor, beta receptor II (70-80kD)
• Transforming growth factor-beta receptor type II

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.