TGF-beta RI/ALK-5 Antibody

Images

 
Western Blot: TGF-beta RI/ALK-5 Antibody [NBP2-20611] - TGF beta Receptor I Antibody [NBP2-20611] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TGF beta Receptor I Antibody [NBP2-20611] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TGF-beta RI/ALK-5 Antibody [NBP2-20611] - Paraffin-embedded mouse intestine. TGF beta Receptor I antibody dilution: 1:500.
Flow Cytometry: TGF-beta RI/ALK-5 Antibody [NBP2-20611] - HepG2 cell fixed in 4% paraformaldehyde at 4-C for 15 minutes. Black: Unlabelled sample was used as a control. Red: TGF beta Receptor I antibody dilution: 1:50. ...read more
Western Blot: TGF-beta RI/ALK-5 Antibody [NBP2-20611] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGE TGF beta Receptor I antibody dilution: 1:10000
Western Blot: TGF-beta RI/ALK-5 Antibody [NBP2-20611] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta Receptor I antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: TGF beta Receptor I Antibody [NBP2-20611] - Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-20611
    • Availability
      Product Discontinued

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TGF-beta RI/ALK-5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TGF beta Receptor I. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Predicted Species
Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TGFBR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry 1:1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Cerebral Meninges Lysate (NB820-60575)

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
2.31 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TGF-beta RI/ALK-5 Antibody

  • AAT5
  • activin A receptor type II-like kinase, 53kD
  • activin A receptor type II-like kinase, 53kDa
  • Activin receptor-like kinase 5
  • ACVRLK4
  • ALK-5
  • ALK-5ALK5
  • EC 2.7.11
  • EC 2.7.11.30
  • LDS1A
  • LDS2A
  • Serine/threonine-protein kinase receptor R4
  • SKR4
  • tbetaR-I
  • TGFB1R1
  • TGF-beta receptor type I
  • TGF-beta receptor type-1
  • TGF-beta RI
  • TGF-beta type I receptor
  • TGFbetaRI
  • TGFBR1
  • TGF-bRI
  • TGFR-1
  • transforming growth factor beta receptor I
  • transforming growth factor, beta receptor 1
  • transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
  • Transforming growth factor-beta receptor type I

Background

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGFBR1
Entrez