TGF-beta RI/ALK-5 Antibody (141231R) [Unconjugated]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 141231R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TGF-beta RI/ALK-5 Antibody (141231R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
  Ala21-Glu121
  Accession # BAA05023
• Specificity: Detects mouse TGF‑ beta RI/ALK-5 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RI/ALK-5 Antibody (141231R) [Unconjugated]

• AAT5
• activin A receptor type II-like kinase, 53kD
• activin A receptor type II-like kinase, 53kDa
• Activin receptor-like kinase 5
• ACVRLK4
• ALK-5
• ALK-5ALK5
• EC 2.7.11
• EC 2.7.11.30
• LDS1A
• LDS2A
• Serine/threonine-protein kinase receptor R4
• SKR4
• tbetaR-I
• TGFB1R1
• TGF-beta receptor type I
• TGF-beta receptor type-1
• TGF-beta RI
• TGF-beta type I receptor
• TGFbetaRI
• TGFBR1
• TGF-bRI
• TGFR-1
• transforming growth factor beta receptor I
• transforming growth factor, beta receptor 1
• transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
• Transforming growth factor-beta receptor type I

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • BAA05023()