Human TGF-beta 2 ELISA Kit (Colorimetric) Summary
| Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human TGF beta 2 positive serum. There was no crossreactivity detected, notably not with human TGF beta 1 and human TGF beta 3. |
| Standard Curve Range |
31.0 - 1,000 pg/mL |
| Sensitivity |
6.6 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10% |
| Intra-Assay |
CV% < 10% |
| Spike Recovery |
The overall mean recovery was > 90%. Please see protocol for more specific Spike Recovery details. |
| Sample Volume |
100 uL (1:50 prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TGFB2 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The human TGF- beta2 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TGF-beta2 |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human TGF-beta2
- Assay Buffer Concentrate 10x
- Biotin-Conjugate anti-human TGF-beta2 monoclonal antibody
- Human TGF-beta2 Standard, 1 ng/ml
- Stop Solution (0.5M H2SO4)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 40x
|
Alternate Names for Human TGF-beta 2 ELISA Kit (Colorimetric)
Background
TGF- beta2 is a potent cytokine which has been shown to modulate embryonic development, bone formation, mammary development, wound healing, hematopoiesis, cell cycle progression and the production of the extracellular matrix. TGF- beta2 -null mice were shown to exhibit perinatal mortality and a wide range of developmental defects for a single gene description which include cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenitial defects. TGF- beta2 has been shown to be a potent growth inhibit factor of uveal melanocytes. It has been described as a factor in the regulation of postnatal cerebellar neurons and neuroblast proliferation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for TGF-beta 2 ELISA Kit (NBP1-91255). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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