TGF-beta 1 Antibody (TB21)

Product Discontinued

TGF-beta 1 Antibody (TB21) Summary

• Immunogen: The antibody reacts with the TGF beta 2.
• Specificity: Recognizes both human platelet-derived and recombinant TGF-beta1 in enzymelinked immunosorbent assay (ELISA). Clone TB21 demonstrates neutralising activity against TGF-beta1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays. Clone TB21 has been demonstrated to react with dimeric (25kD) or monomeric (12.5kD) molecules of natural TGF-beta1 under non-reducing and reducing conditions respectively. Clone TB21 has been reported as being suitable for use in flow cytometry with permeabilisation (1). Cross reactive with sheep, rat, mustelied Histology positive control tissue: Human breast carcinoma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: This product does not require protein digestion pre-treatment of paraffin sections. Paraformaldehyde fixation recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Notes

This product should be stored undiluted.

Alternate Names for TGF-beta 1 Antibody (TB21)

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFB1
• Entrez:
  • 7042(Human)
• Uniprot:
  • P61812()