TGF-beta 1 Antibody (9016) [Unconjugated]

TGF-beta 1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-TGF-beta 1 Monoclonal Antibody (Catalog # MAB240) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TGF-beta 1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti- TGF-beta 1 Monoclonal Antibody (Catalog # MAB240) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in the prostate gland. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240). The ND50 is typically 0.3-1.0 µg/mL.

ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

A33+ Li-EVs alleviate IBD through TGF-beta 1 signalling.(a) A33+ Li-EVs were isolated from healthy control or 2% DSS-induced IBD mice. TGF-beta 1, A33 and beta -Actin were detected by western blot analysis. (b) The CD4+ T-cell proliferation assay was performed as described above in the presence of 30 μg ml−1 Ctrl or IBD-A33+ Li-EVs, and the results were statistically analysed (n=9). (c–f) Mice were fed with drinking water containing 2% DSS on day 0. On days −2 and 2, the mice were intravenously treated with the indicated dose of A33+ Li-EVs. The body weights were measured daily (c). Appearance (left) and statistical analysis (right) of colonic length on days 11 (d). Histological appearance on day 11. Representative colonic sections stained with haematoxylin and eosine (H&E). In the PBS group, the right image is a magnified region of the left image (e). IL-1 beta , IL-6, TNF-alpha , IL-10, IL-22 levels and MPO activity in colon tissue were measured on day 11 (f). (g) IBD mice were treated with 100 μg A33+ Li-EVs, IBD-A33+ Li-EVs and Sp-EVs on days −2 and 2. The body weights were measured daily. (h) TGF-beta 1 in A33+ Li-EVs and Sp-EVs was detected using western blot analysis. (i) IBD was introduced into Smad3+/− mice and treated with 100 μg A33+ Li-EVs and IBD-A33+ Li-EVs on days −2 and 2. The body weights were measured daily. Ctrl group, mice received normal drinking water; PBS group, mice with drinking water containing 2% DSS and intravenously treated with PBS on days −2 and 2. (a,b,d–f) Data are representative of three independent experiments or shown as mean values±s.e.m. pooled from three independent experiments; (c,g,i) data are presented as the mean±s.d. from one of the three independent experiments (n=5 per group). P values were generated by one-way ANOVA in b,d,f, or two-way ANOVA in c,g,i, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (*P<0.05, **P<0.01, ***P<0.001), corresponding colour indicating the relevant group versus PBS group in c, versus PBS group in d,f; IBD-A33+ Li-EVs versus A33+ Li-EVs in g. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

CD4+ Vδ1+ T-cell clones have a TH0-cytokine profile and are effector-memory cells. (A) CD4+ Vδ1+ clones produce regulatory IL-10 and TGF-beta , TH2 cytokines IL-4, -5, and -13, and to a lesser extent proinflammatory IL-17A and IL-6, and the TH1-cytokines IL-2, IFN-gamma , and TNF-alpha after stimulation with PMA/ionomycin. (B) CD4+ Vδ1+ clones were not naïve but belonged to the effector memory; they were CD45RA−, CD27−, CCR7−, and predominantly CD62L− and CD28±. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2014.00645/abstract), licensed under a CC-BY license. Not internally tested by R&D Systems.

ERK and TGF-beta 1 levels in IBD-A33+ Li-EVs.(a) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38 cells or MC38-EVs was measured using western blot analysis. (b) MC38 cells were treated with 1 mg ml−1 IBD lysates or Ctrl lysates for the indicated times. Activation of ERK, JNK, p38 and ATK in MC38 cells was measured using western blot analysis. (c) MC38 cells were pretreated with DMSO, 10 μM ERK-specific inhibitor U0126 or JNK-specific inhibitor SP600125 for 30 min and then treated with 1 mg ml−1 IBD lysates or Ctrl lysates for 24 h. TGF-beta 1 in MC38-EVs was measured by western blot analysis. (d) Activation of ERK, JNK, p38 and ATK in colon tissues from healthy control or IBD mice was measured using western blot analysis. (e,f) Mice were fed with 2% DSS solution on day 0. Mice received intrarectal injection with 20 μg cholesterol-conjugated ERK or NC siRNA every other day on days 0–11. Twenty-four hours after the last injection, mice were killed. TGF-beta 1 in colon tissues and A33+ Li-EVs was measured using western blot analysis (e). The body weights of mice were measured daily (f). Ctrl group, mice that received normal drinking water. (a–e) Data are representative of three independent experiments; (f) data are presented as the mean±s.d. from one of the two independent experiments (n=5 per group). P values were generated by two-way ANOVA, followed by Newman–Keuls multiple comparison test using GraphPad Prism 5 (**P<0.01, ***P<0.001), IBD+NC siRNA versus IBD+ERK siRNA. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27721471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Discontinued

TGF-beta 1 Antibody (9016) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
• Specificity: Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than
  2% cross-reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.3-1.0 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: TGF-beta 1 Biotinylated Antibody (Catalog number BAF240)
  Standard: Recombinant Human TGF-beta 1 (Catalog number 240-B)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 1 Antibody (9016) [Unconjugated]

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1 Antibody (MAB240)(89)

Publications using MAB240

• Lin, HY;Lu, JH;Lin, RJ;Chueh, KS;Juan, TJ;Mao, JW;Lee, YC;Chuang, SM;Shen, MC;Sun, TW;Juan, YS; Effects of Nitric Oxide on Bladder Detrusor Overactivity through the NRF2 and HIF-1? Pathways: A Rat Model Induced by Metabolic Syndrome and Ovarian Hormone Deficiency International journal of molecular sciences 2024-10-16 [PMID: 39456884] (Western Blot, Rat)
• J Manokawinc, S Chareonvit, V Trachoo, P Limraksasi, H Egusa, T Osathanon Intermittent compressive force regulates dentin matrix protein 1 expression in human periodontal ligament stem cells Journal of dental sciences, 2022-07-15;18(1):105-111. 2022-07-15 [PMID: 36643268] (Neutralization, Human)
• R Wang, AB Bhatt, BA Minden-Bir, OK Travis, S Tiwari, H Jia, W Rosikiewic, O Martinot, E Childs, R Loesch, G Tossou, S Jamieson, D Finkelstei, B Xu, M Labelle ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis Science Advances, 2023-01-06;9(1):eabq3951. 2023-01-06 [PMID: 36608120] (Neutralization, Human)
• T Ko, S Nomura, S Yamada, K Fujita, T Fujita, M Satoh, C Oka, M Katoh, M Ito, M Katagiri, T Sassa, B Zhang, S Hatsuse, T Yamada, M Harada, H Toko, E Amiya, M Hatano, O Kinoshita, K Nawata, H Abe, T Ushiku, M Ono, M Ikeuchi, H Morita, H Aburatani, I Komuro Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-beta-IGFBP7 axis Nature Communications, 2022-06-07;13(1):3275. 2022-06-07 [PMID: 35672400] (In Vivo, Mouse)
• H Yang, S Berezowska, P Dorn, P Zens, P Chen, RW Peng, TM Marti, GJ Kocher, RA Schmid, SRR Hall Tumor-infiltrating lymphocytes are functionally inactivated by CD90+ stromal cells and reactivated by combined Ibrutinib and Rapamycin in human pleural mesothelioma Theranostics, 2022-01-01;12(1):167-185. 2022-01-01 [PMID: 34987640] (Neutralization, Human)
• L Gu, R Surolia, JL Larson-Cas, C He, D Davis, J Kang, VB Antony, AB Carter Targeting Cpt1a-Bcl-2 interaction modulates apoptosis resistance and fibrotic remodeling Cell Death and Differentiation, 2021-08-20;0(0):. 2021-08-20 [PMID: 34413485] (Western Blot, Mouse)
• KM McAndrews, K Vázquez-Ar, C Kwak, H Sugimoto, X Zheng, B Li, ML Kirtley, VS LeBleu, R Kalluri &amp;alphaSMA+ fibroblasts suppress Lgr5+ cancer stem cells and restrain colorectal cancer progression Oncogene, 2021-06-09;0(0):. 2021-06-09 [PMID: 34108617] (IHC, Mouse)
• A Shi, J Li, X Qiu, M Sabbah, S Boroumand, TC Huang, C Zhao, A Terzic, A Behfar, SL Moran TGF-&amp;beta loaded exosome enhances ischemic wound healing in vitro and in vivo Theranostics, 2021-04-30;11(13):6616-6631. 2021-04-30 [PMID: 33995680] (IHC, Rabbit)
• SS Chiang, LS Chen, CY Chu Active food ingredients production from cold pressed processing residues of Camellia oleifera and Camellia sinensis seeds for regulation of blood pressure and vascular function Chemosphere, 2020-12-11;267(0):129267. 2020-12-11 [PMID: 33338714] (IHC, Mouse)
• S Matsui, N Yoneda, H Maezono, K Kuremoto, T Ishimoto, T Nakano, H Yumoto, S Ebisu, Y Noiri, M Hayashi Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats PLoS ONE, 2020-09-29;15(9):e0239660. 2020-09-29 [PMID: 32991601] (IHC, Rat)
• LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (SEM, Human)
• A Haddad, M Gaudet, M Plesa, Z Allakhverd, AK Mogas, S Audusseau, CJ Baglole, DH Eidelman, R Olivenstei, MS Ludwig, Q Hamid Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells Respir. Res., 2019-10-29;20(1):234. 2019-10-29 [PMID: 31665016] (ICC, Neutralization, Human)
• P Zhao, B Wang, Z Zhang, W Zhang, Y Liu Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression Cell Death Dis, 2019-10-10;10(10):776. 2019-10-10 [PMID: 31601783] (Neutralization, Human)
• J Manokawinc, P Pavasant, C Sawangmake, N Limjeeraja, CN Limjeeraja, H Egusa, T Osathanon Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-beta pathway Cell Death Dis, 2019-10-07;10(10):761. 2019-10-07 [PMID: 31591384] (ICC, Neutralization, Human)
• X Zang, L He, L Zhao, Y He, E Xiao, Y Zhang Adipose-derived stem cells prevent the onset of bisphosphonate-related osteonecrosis of the jaw through transforming growth factor ?-1-mediated gingival wound healing Stem Cell Res Ther, 2019-06-13;10(1):169. 2019-06-13 [PMID: 31196208] (Western Blot, Human)
• KN Khan, K Yamamoto, A Fujishita, A Koshiba, H Kuroboshi, S Sakabayash, S Teramukai, M Nakashima, J Kitawaki Association between FOXP3+ regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts Reprod. Biomed. Online, 2019-01-31;0(0):. 2019-01-31 [PMID: 30981619] (IHC-P, Human)
• CL Lin, YC Hsu, YT Huang, YH Shih, CJ Wang, WC Chiang, PJ Chang A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction EMBO Mol Med, 2019-05-01;11(5):. 2019-05-01 [PMID: 30948420] (Neutralization, Mouse)
• M McMillin, S Grant, G Frampton, AD Petrescu, E Williams, B Jefferson, A Thomas, A Brahmarout, S DeMorrow Elevated circulating TGF?1 during acute liver failure activates TGF?R2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice J Neuroinflammation, 2019-04-02;16(1):69. 2019-04-02 [PMID: 30940161] (Neutralization, Mouse)
• Y Jiang, F You, J Zhu, C Zheng, R Yan, J Zeng Cryptotanshinone Ameliorates Radiation-Induced Lung Injury in Rats Evid Based Complement Alternat Med, 2019-02-20;2019(0):1908416. 2019-02-20 [PMID: 30915142] (IHC-P, Rat)
• L Buscemi, M Price, P Bezzi, L Hirt Spatio-temporal overview of neuroinflammation in an experimental mouse stroke model Sci Rep, 2019-01-24;9(1):507. 2019-01-24 [PMID: 30679481] (IHC, Mouse)
• S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge Nat Commun, 2018-09-24;9(1):3879. 2018-09-24 [PMID: 30250029]
• CS Abdullah, ZQ Jin Targeted deletion of T cell S1P receptor 1 ameliorates cardiac fibrosis in streptozotocin-induced diabetic mice FASEB J., 2018-04-26;0(0):fj201800231R. 2018-04-26 [PMID: 29698062] (IHC-P, Mouse)
• X Ji, H Wang, Z Wu, X Zhong, M Zhu, Y Zhang, R Tan, Y Liu, J Li, L Wang Specific Inhibitor of Smad3 (SIS3) Attenuates Fibrosis, Apoptosis, and Inflammation in Unilateral Ureteral Obstruction Kidneys by Inhibition of Transforming Growth Factor ? (TGF-?)/Smad3 Signaling Med. Sci. Monit., 2018-03-20;24(0):1633-1641. 2018-03-20 [PMID: 29555895] (Western Blot, Mouse)
• S Wan, S Wang, L Weng, G Zhang, Z Lin, X Fei, F Zhang, F Yang, J Wang, Z Cai CD8?+CD11c+ Extracellular Vesicles in the Lungs Control Immune Homeostasis of the Respiratory Tract via TGF-?1 and IL-10 J. Immunol., 2018-01-22;0(0):. 2018-01-22 [PMID: 29358274] (Functional Assay, Neutralization, Mouse)
• G Wang, F Zhao, D Yang, J Wang, L Qiu, X Pang Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGF?1 and microRNA-34a-5p Int. J. Mol. Med., 2017-11-17;41(2):791-799. 2017-11-17 [PMID: 29207015] (Neutralization, Human)
• T Shimizu, N Narang, P Chen, B Yu, M Knapp, J Janardanan, J Blair, JK Liao Fibroblast deletion of ROCK2 attenuates cardiac hypertrophy, fibrosis, and diastolic dysfunction JCI Insight, 2017-07-06;2(13):. 2017-07-06 [PMID: 28679962] (Neutralization, Rat)
• JH Park, B Park, KK Park Suppression of Hepatic Epithelial-to-Mesenchymal Transition by Melittin via Blocking of TGF?/Smad and MAPK-JNK Signaling Pathways Toxins (Basel), 2017-04-13;9(4):. 2017-04-13 [PMID: 28406452] (IHC-P, Mouse)
• C Zhang, J Lim, J Liu, B Ponugoti, S Alsadun, C Tian, R Vafa, DT Graves FOXO1 expression in keratinocytes promotes connective tissue healing Sci Rep, 2017-02-21;7(0):42834. 2017-02-21 [PMID: 28220813] (Neutralization, Human)
• RJ Moritz, RG LeBaron, CF Phelix, R Rupaimoole, HS Kim, A Tsin, R Asmis Macrophage TGF-?1 and the Proapoptotic Extracellular Matrix Protein BIGH3 Induce Renal Cell Apoptosis in Prediabetic and Diabetic Conditions Int J Clin Med, 2016-07-21;7(7):496-510. 2016-07-21 [PMID: 28149671] (Western Blot, Human)
• O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2016-12-27;45(4):945-953. 2016-12-27 [PMID: 28027451] (Western Blot, Human)
• A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 2016-11-22;7(0):13363. 2016-11-22 [PMID: 27874859] (Western Blot, Mouse)
• Jiping Qi Role of urotensin�II in advanced glycation end product-induced extracellular matrix synthesis in rat proximal tubular epithelial cells Int. J. Mol. Med., 2016-10-26;0(0):. 2016-10-26 [PMID: 27840897] (Neutralization, Rat)
• Platelet-Derived Ectosomes Reduce NK Cell Function J Immunol, 2016-07-22;0(0):. 2016-07-22 [PMID: 27448586] (Neutralization, Human)
• Brian G Oliver A novel immunomodulatory function of neutrophils on rhinovirus-activated monocytes in vitro Thorax, 2016-06-10;71(11):1039-1049. 2016-06-10 [PMID: 27287090] (Neutralization, Human)
• Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis. Nat Commun, 2016-03-18;7(0):11012. 2016-03-18 [PMID: 26987776] (Western Blot, Human)
• GM Bove, MY Harris, H Zhao, MF Barbe Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury J. Neurol. Sci, 2015-12-24;361(0):168-80. 2015-12-24 [PMID: 26810536] (IHC-P, Rat)
• Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol, 2015-07-31;195(5):2141-8. 2015-07-31 [PMID: 26232426] (Neutralization, Human)
• Sommer A, Fabri M Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 2015-06-24;10(6):e0130395. 2015-06-24 [PMID: 26107738] (Neutralization, Human)
• Pahlman L, Jogi A, Gram M, Mori M, Egesten A Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1. BMC Pulm Med, 2015-03-07;15(0):19. 2015-03-07 [PMID: 25851169] (Neutralization, Human)
• Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65. 2015-02-17 [PMID: 25730867] (Flow Cytometry, Mouse)
• Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses. Nat Commun, 2015-02-19;6(0):6329. 2015-02-19 [PMID: 25695838] (Neutralization, Mouse)
• Ogawa H, Ledford J, Mukherjee S, Aono Y, Nishioka Y, Lee J, Izumi K, Hollingsworth J Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-ss Respir Res, 2014-11-29;15(1):143. 2014-11-29 [PMID: 25472740] (Neutralization, Mouse)
• Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination. Eur J Immunol, 2015-02-01;45(2):442-51. 2015-02-01 [PMID: 25141829] (Neutralization, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36. 2014-05-13 [PMID: 24825889] (Immunoprecipitation, Human)
• Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y Mesenchymal stem cells cancel azoxymethane-induced tumor initiation. Stem Cells, 2014-04-01;32(4):913-25. 2014-04-01 [PMID: 24715689] (Neutralization, Rat)
• Qu X, Li X, Zheng Y, Ren Y, Puelles V, Caruana G, Nikolic-Paterson D, Li J Regulation of renal fibrosis by Smad3 Thr388 phosphorylation. Am J Pathol, 2014-01-30;184(4):944-52. 2014-01-30 [PMID: 24485922] (Neutralization, Rat)
• Miller M, Petty C, Tompkins M, Fogle J CD4+CD25+ T regulatory cells activated during feline immunodeficiency virus infection convert T helper cells into functional suppressors through a membrane-bound TGFbeta / GARP-mediated mechanism. Virol J, 2014-01-18;11(0):7. 2014-01-18 [PMID: 24438223] (Neutralization, Feline)
• Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. J Immunol, 2013-12-11;192(2):804-16. 2013-12-11 [PMID: 24337375] (ELISA Development, Mouse)
• Bialas A, Stevens B TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement. Nat Neurosci, 2013-10-27;16(12):1773-82. 2013-10-27 [PMID: 24162655] (Immunodepletion, Mouse)
• Hasegawa T, Yashiro M, Nishii T, Matsuoka J, Fuyuhiro Y, Morisaki T, Fukuoka T, Shimizu K, Shimizu T, Miwa A, Hirakawa K Cancer-associated fibroblasts might sustain the stemness of scirrhous gastric cancer cells via transforming growth factor-beta signaling. Int J Cancer, 2013-10-24;134(8):1785-95. 2013-10-24 [PMID: 24155219] (Neutralization, Human)
• Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction. Eur J Immunol, 2013-06-21;43(9):2461-72. 2013-06-21 [PMID: 23716181] (Neutralization, Mouse)
• Rodrigues T, Matafome P, Santos-Silva D, Sena C, Seica R Reduction of methylglyoxal-induced glycation by pyridoxamine improves adipose tissue microvascular lesions. J Diabetes Res, 2013-04-07;2013(0):690650. 2013-04-07 [PMID: 23671887] (IHC, Western Blot, Rat)
• Guo Z, Khattar M, Schroder P, Miyahara Y, Wang G, He X, Chen W, Stepkowski S A dynamic dual role of IL-2 signaling in the two-step differentiation process of adaptive regulatory T cells. J Immunol, 2013-02-20;190(7):3153-62. 2013-02-20 [PMID: 23427250] (Neutralization, Mouse)
• Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, Zou B, Xie X, Pasricha P Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis. Mol Pain, 2012-09-11;8(0):65. 2012-09-11 [PMID: 22963239] (Neutralization, Rat)
• Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages. PLoS ONE, 2012-03-06;7(3):e32761. 2012-03-06 [PMID: 22412921] (Neutralization, Human)
• Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. J. Clin. Invest., 2011-10-17;121(11):4548-66. 2011-10-17 [PMID: 22005303] (Western Blot, Human)
• Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011-07-09;54(10):2713-23. 2011-07-09 [PMID: 21744073] (ELISA Development, Human)
• Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol. Biol. Cell, 2011-03-16;22(10):1686-98. 2011-03-16 [PMID: 21411626] (Neutralization, Canine)
• Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity. J. Biol. Chem., 2011-03-11;286(20):17435-44. 2011-03-11 [PMID: 21398522] (Neutralization, Mouse)
• Oida T, Weiner HL Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J. Immunol., 2010-08-18;185(6):3529-35. 2010-08-18 [PMID: 20720212] (Flow Cytometry, Human)
• Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection. J. Immunol., 2009-12-16;184(2):984-92. 2009-12-16 [PMID: 20018630] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))
• Pot C, Jin H, Awasthi A Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells. J. Immunol., 2009-07-01;183(2):797-801. 2009-07-01 [PMID: 19570826] (Neutralization, Mouse)
• Meltendorf C, Burbach GJ, Ohrloff C, Ghebremedhin E, Deller T Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction. Invest. Ophthalmol. Vis. Sci., 2009-04-22;50(8):3688-95. 2009-04-22 [PMID: 19387066] (IHC-Fr, Rabbit)
• Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation. J. Exp. Med., 2009-03-30;206(4):819-31. 2009-03-30 [PMID: 19332876] (ICC, Western Blot, Mouse)
• Bhowmick S, Mazumdar T, Ali N Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection. Infect. Immun., 2009-01-21;77(4):1514-23. 2009-01-21 [PMID: 19168736] (Neutralization, Mouse)
• Cerboni C, Ardolino M, Santoni A, Zingoni A Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 2009-01-05;113(13):2955-64. 2009-01-05 [PMID: 19124832] (Neutralization, Human)
• Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M Activation of Toll-like receptor 3 augments myofibroblast differentiation. Am. J. Respir. Cell Mol. Biol., 2008-11-06;40(6):654-62. 2008-11-06 [PMID: 18988918] (ELISA Development, Neutralization, Human)
• Walsh MF, Ampasala DR, Hatfield J, Vander Heide R, Suer S, Rishi AK, Basson MD Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via smad- and p38-dependent mechanisms. Am. J. Pathol., 2008-06-26;173(2):385-99. 2008-06-26 [PMID: 18583311] (ICC, IHC-P, Human, Rat)
• Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells, 2008-01-10;26(4):1047-55. 2008-01-10 [PMID: 18192235] (ELISA Development, Rat)
• Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection. J. Immunol., 2008-01-01;180(1):113-21. 2008-01-01 [PMID: 18097010] (Neutralization, Mouse)
• Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 2007-08-05;8(9):942-9. 2007-08-05 [PMID: 17676045] (Neutralization, Human)
• Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Western Blot, Human)
• Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. J. Immunol., 2007-05-01;178(9):5552-62. 2007-05-01 [PMID: 17442937] (Neutralization, Human)
• Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J. Immunol., 2007-02-15;178(4):2018-27. 2007-02-15 [PMID: 17277105] (Neutralization, Mouse)
• Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA Increased frequency of CD4+ cells expressing CD161 in cancer patients. Clin. Cancer Res., 2006-12-01;12(23):6901-9. 2006-12-01 [PMID: 17145807] (Neutralization, Human)
• Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Blood, 2006-11-02;109(5):2234-42. 2006-11-02 [PMID: 17082320] (Array Development, Mouse)
• Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy. Am. J. Pathol., 2006-11-01;169(5):1527-40. 2006-11-01 [PMID: 17071578] (IHC-P, Mouse)
• Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006-08-17;241(1):7-13. 2006-08-17 [PMID: 16914126] (Neutralization, Human)
• Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma. J. Hepatol., 2006-03-09;45(2):254-62. 2006-03-09 [PMID: 16600416] (Neutralization, Human)
• Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression. Immunol. Lett., 2006-03-03;105(2):159-66. 2006-03-03 [PMID: 16564576] (ELISA Development, Human)
• Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-12-01;114(11):1640-9. 2004-12-01 [PMID: 15578096] (Neutralization, Human)
• Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 2004-09-16;105(2):750-8. 2004-09-16 [PMID: 15374887] (Neutralization, Human)
• Phillips TM Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Electrophoresis, 2004-06-01;25(10):1652-9. 2004-06-01 [PMID: 15188254] (Affinity Chromatography, Human)
• Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription. J. Biol. Chem., 2002-09-16;277(47):45547-57. 2002-09-16 [PMID: 12237307] (Neutralization, Human)
• Schmidt A, 2019, Gopfert C, Vlodavsky I, Volker W, Buddecke E Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta. 101211, 2002-03-01;81(3):138-44. 2002-03-01 [PMID: 11998865] (Neutralization, Bovine)
• Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56. 2002-05-01 [PMID: 11943670] (ELISA Development, Human)
• Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4. J. Immunol., 2000-05-15;164(10):5337-43. 2000-05-15 [PMID: 10799896] (ELISA Development, Primate - Papio anubis (Olive Baboon))
• Mozes MM, Hodics T, Kopp JB Isoform specificity of commercially-available anti-TGF-beta antibodies. J. Immunol. Methods, 1999-05-27;225(1):87-93. 1999-05-27 [PMID: 10365785] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TGFB1