TFCP2L1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

TFCP2L1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TFCP2L1
Met1-Lys180
Accession # Q9NZI6
Specificity
Detects human TFCP2L1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TFCP2L1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5726 in the following application:

Publications
Read Publications using
AF5726 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFCP2L1 Antibody [Unconjugated]

  • CRTR1
  • CRTR1CRTR-1
  • LBP9
  • LBP-9
  • LBP9CP2-related transcriptional repressor 1
  • TFCP2L1
  • transcription factor CP2-like 1
  • transcription factor CP2-like protein 1
  • Transcription factor LBP-9

Background

TFCP2L1 (Transcription factor CP2‑like protein # 1; also LBP‑9) is a 54 kDa (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP‑1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100‑200) and a dimerization domain (aa 300‑479). There is one splice variant that shows a deletion of aa 400‑464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR‑1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TFCP2L1 Antibody (AF5726)(5)

We have publications tested in 2 confirmed species: Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 3 applications: ICC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(2)
Immunoprecipitation
(1)
Western Blot
(2)
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Mouse
(4)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
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Showing Publications 1 - 5 of 5.
Publications using AF5726 Applications Species
DC Wan, SL Morgan, AL Spencley, N Mariano, EY Chang, G Shankar, Y Luo, TH Li, D Huh, SK Huynh, JM Garcia, CM Dovey, J Lumb, L Liu, KV Brown, A Bermudez, R Luong, H Zeng, VL Mascetti, SJ Pitteri, J Wang, H Tu, M Quarta, V Sebastiano, R Nusse, TA Rando, JE Carette, JF Bazan, KC Wang Honey bee Royalactin unlocks conserved pluripotency pathway in mammals Nat Commun, 2018;9(1):5078. 2018 [PMID: 30510260] (Western Blot, Mouse) Western Blot Mouse
Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys Cell Rep, 2018;25(9):2563-2576.e9. 2018 [PMID: 30485820] (ICC, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) ICC Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30220521] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
S Rajderkar, C Panaretos, V Kaartinen Trim33 regulates early maturation of mouse embryoid bodies in vitro Biochem Biophys Rep, 2017;12(0):185-192. 2017 [PMID: 29090280] (Western Blot, Mouse) Western Blot Mouse
Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J alpha-Intercalated cells defend the urinary system from bacterial infection. J Clin Invest, 2014;124(7):2963-76. 2014 [PMID: 24937428] (ICC, Mouse) ICC Mouse

Review for TFCP2L1 Antibody (AF5726) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF5726:
Filter by Applications
ICC
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TFCP2L1 AF5726
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reviewed by:
Anonymous
ICC Mouse 08/04/2016
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Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotCCUG0115021

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:300, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Details

Detection NotesFluorescence Imaging, AF488 with EGFP filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol TFCP2L1
Uniprot