RBM27 Antibody - BSA Free

Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human and Mouse RBM27 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: RBM27 Antibody [NB100-60426] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RBM27 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: RBM27 Antibody [NB100-60426] - Detection of Human RBM27 on HeLa whole cell lysate using NB100-60426. RBM27 was also immunoprecipitated by rabbit anti-RBM27 antibody NB100-60427.

Immunoprecipitation: RBM27 Antibody [NB100-60426] - Detection of human RBM27 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM27 antibody NB100-60426 used for IP at 6 ug per reaction. RBM27 was also immunoprecipitated by another rabbit anti-RBM27 antibody. For blotting immunoprecipitated RBM27, NB100-60426 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RBM27 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBM27 Antibody - BSA Free (NB100-60426) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human RNA binding motif protein 27 using the numbering given in entry Q9P2N5.2 (GeneID 54439).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBM27 Antibody - BSA Free

• RNA-binding protein 27
• ARRS1
• Psc1
• RNA binding motif protein 27

Background

RBM27 (RNA-binding motif protein 27) contains a C3H1-type zinc-finger and RNA recognition motif domain. The function of RBM27 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM27
• Entrez:
  • 54439(Human)
• Uniprot:
  • Q9P2N5.2(Human)