TER-119 Antibody (TER-119) [Biotin]

TER-119 was detected in immersion fixed frozen sections of mouse embryo (E15) using Rat Anti-Mouse TER-119 Biotinylated Monoclonal Antibody (Catalog # BAM1125) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to red blood cell plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP
• Clone: TER-119
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

TER-119 Antibody (TER-119) [Biotin] Summary

• Immunogen: C57BL/6 mouse day-14 fetal liver cells
• Specificity: Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly76
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TER-119 Antibody (TER-119) [Biotin]

• Ly76
• Ly-76
• TER119
• TER-119

Background

The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TER-119 Antibody (BAM1125)(2)

Publications using BAM1125

• Ke, X, Do, D C Et al. Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. J Allergy Clin Immunol 4/1/2019 [PMID: 30194990]
• Zhang DL, Senecal T, Ghosh MC Hepcidin regulates ferroportin expression and intracellular iron homeostasis of erythroblasts. Blood, 2011-06-23;118(10):2868-77. 2011-06-23 [PMID: 21700773] (Immunoprecipitation, Mouse)

Bioinformatics

• Gene Symbol: Ly76