TER-119 Antibody (TER-119) [Phycoerythrin]

Balb/c mouse bone marrow cells was stained with Rat Anti-Mouse TER-119 PE-conjugated Monoclonal Antibody (Catalog # FAB1125P) and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). Quadrant markers were set based on control antibody staining . View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: TER-119
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

TER-119 Antibody (TER-119) [Phycoerythrin] Summary

• Immunogen: C57BL/6 mouse day-14 fetal liver cells
• Specificity: Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly76
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TER-119 Antibody (TER-119) [Phycoerythrin]

• Ly76
• Ly-76
• TER119
• TER-119

Background

The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).

1. Kina, T. et al. (2000) Br. J. Haematol. 109:280.
2. Ikuta, K. et al. (1990) Cell 62:863.
3. Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TER-119 Antibody (FAB1125P)(2)

Publications using FAB1125P

• L Yang, P Shivakumar, JM Kinder, SS Way, B Donnelly, R Mourya, Z Luo, JA Bezerra Regulation of bile duct epithelial injury by hepatic CD71+ erythroid cells JCI Insight, 2020-06-04;0(0):. 2020-06-04 [PMID: 32407296] (Flow Cytometry, Mouse)
• Johns JL, Macnamara KC, Walker NJ, Winslow GM, Borjesson DL Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells. Infect. Immun., 2009-06-29;77(9):4070-80. 2009-06-29 [PMID: 19564373] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Ly76