TER-119 Antibody (TER-119) [Janelia Fluor® 669]

• Reactivity: Mu
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: TER-119
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 669

TER-119 Antibody (TER-119) [Janelia Fluor® 669] Summary

• Immunogen: C57BL/6 mouse day-14 fetal liver cells
• Specificity: Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly76
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TER-119 Antibody (TER-119) [Janelia Fluor® 669]

• Ly76
• Ly-76
• TER119
• TER-119

Background

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E. The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ly76