TER-119 Antibody (TER-119) [Allophycocyanin/Cy7]

• Reactivity: Mu
• Applications: Flow
• Clone: TER-119
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin/Cy7

TER-119 Antibody (TER-119) [Allophycocyanin/Cy7] Summary

• Immunogen: C57BL/6 mouse day-14 fetal liver cells
• Specificity: Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly76
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for TER-119 Antibody (TER-119) [Allophycocyanin/Cy7]

• Ly76
• Ly-76
• TER119
• TER-119

Background

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E. The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ly76